Immunogenicity of multi-epitope-based vaccine candidates administered with the adjuvant Gp96 against rabies

文献类型： 外文期刊

第一作者： Niu, Yange

作者： Niu, Yange;Yang, Limin;Qu, Hongren;Li, Jing;Liu, Wenjun;Niu, Yange;Liu, Wenjun;Liu, Ye;Hu, Rongliang;Liu, Ye;Hu, Rongliang;Zhao, Jingyi;Li, Jing

作者机构：

关键词： rabies virus;multi-epitope-based vaccine;immunogenicity evaluation;Gp96

期刊名称：VIROLOGICA SINICA （ 影响因子：4.327； 五年影响因子：4.08 ）

ISSN： 1674-0769

年卷期： 2016 年 31 卷 2 期

页码：

收录情况： SCI

摘要： Rabies, a zoonotic disease, causes > 55,000 human deaths globally and results in at least 500 million dollars in losses every year. The currently available rabies vaccines are mainly inactivated and attenuated vaccines, which have been linked with clinical diseases in animals. Thus, a rabies vaccine with high safety and efficacy is urgently needed. Peptide vaccines are known for their low cost, simple production procedures and high safety. Therefore, in this study, we examined the efficacy of multi-epitope-based vaccine candidates against rabies virus. The ability of various peptides to induce epitope-specific responses was examined, and the two peptides that possessed the highest antigenicity and conservation, i.e., AR16 and hPAB, were coated with adjuvant canine-Gp96 and used to prepare vaccines. The peptides were prepared as an emulsion of oil in water (O/W) to create three batches of bivalent vaccine products. The vaccine candidates possessed high safety. Virus neutralizing antibodies were detected on the day 14 after the first immunization in mice and beagles, reaching 5-6 IU/mL in mice and 7-9 IU/mL in beagles by day 28. The protective efficacy of the vaccine candidates was about 70%-80% in mice challenged by a virulent strain of rabies virus. Thus, a novel multi-epitope-based rabies vaccine with Gp96 as an adjuvant was developed and validated in mice and dogs. Our results suggest that synthetic peptides hold promise for the development of novel vaccines against rabies.

分类号：

  • 相关文献

[1]Ovine rotavirus strain LLR-85-based bovine rotavirus candidate vaccines: Construction, characterization and immunogenicity evaluation. Chang, Ji-Tao,Li, Xin,Liu, Hai-Jun,Yu, Li.

[2]Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels. Yu, Gui Mei,Zu, Shu Long,Zhou, Wei Wei,Wang, Xi Jun,Shuai, Lei,Wang, Xue Lian,Ge, Jin Ying,Bu, Zhi Gao. 2017

[3]Seroprevalence of Rabies Virus Antibodies in Bats from Southern China. Jiang, Yu,Wang, Lili,Han, Xiaohu,Xia, Xianzhu,Tu, Changchun,Wang, Lili,Zhao, Fuguang,Lu, Zongji,Xuan, Hua. 2010

[4]An inactivated recombinant rabies CVS-11 virus expressing two copies of the glycoprotein elicits a higher level of neutralizing antibodies and provides better protection in mice. Xue, Xiang-Hong,Li, Ling,Yang, Song-Tao,Xia, Xian-Zhu,Xue, Xiang-Hong,Zheng, Xue-Xing,Wang, Hua-Lei,Ma, Jin-Zhu,Li, Ling,Gai, Wei-Wei,Wang, Tie-Cheng,Yang, Song-Tao,Xia, Xian-Zhu,Zheng, Xue-Xing,Ma, Jin-Zhu.

[5]Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge. Wang, Feng-Xue,Zhang, Shu-Qin,Zhu, Hong-Wei,Yang, Yong,Sun, Na,Tan, Bin,Li, Zhen-Guang,Cheng, Shi-Peng,Wen, Yong-Jun,Li, Zhen-Guang,Fu, Zhen F..

[6]Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. Yin, Xiangping,Li, Zhiyong,Li, Jiangtao,Zhang, Yun,Li, Xuerui,Li, Baoyu,Yang, Bin,Lan, Xi,Jiao, Wenqiang,Liu, Jixing,Yi, Yongzhu,Li, Yinv,Zhang, Zhifang.

[7]Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination. Shuai, Lei,Wang, Xijun,Ge, Jinying,Wen, Zhiyuan,Chen, Weiye,Qin, Lide,Bu, Zhigao,Feng, Na,Xia, Xianzhu.

[8]Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production. Tao, Lihong,Ge, Jinying,Wang, Xijun,Wen, Zhiyuan,Zhai, Hongyue,Hua, Tao,Zhao, Bolin,Kong, Dongni,Bu, Zhigao,Yang, Chinglai. 2011

[9]Immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice. Xiao, Xing-xing,Zhang, Yun,Liu, Ji-xing,Wei, Qiao-lin,Yin, Xiang-ping.

[10]Enhancement of blood-brain barrier permeability is required for intravenously administered virus neutralizing antibodies to clear an established rabies virus infection from the brain and prevent the development of rabies in mice. Huang, Chien-Tsun,Li, Zhenguang,Huang, Ying,Zhang, Guoqing,Zhou, Ming,Chai, Qingqing,Fu, Zhen F.,Li, Zhenguang,Wu, Hua,Fu, Zhen F..

[11]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

[12]Engineering, expression, and immuno-characterization of recombinant protein comprising multi-neutralization sites of rabies virus glycoprotein. Li, Xiangdong,Luo, Jinyan,Shen, Yang,Qiu, Yafeng,Wang, Xiaodu,Deng, Xufang,Ding, Chan,Ma, Zhiyong,Wang, Shuiming,Liu, Xuehui,Bao, Weidong,Liu, Peihong,Zhou, Jinping.

[13]Characterization of a virulent dog-originated rabies virus affecting more than twenty fallow deer (Dama dama) in Inner Mongolia, China. Zhu, Hongwei,Shao, Xiqun,Ba, Hengxing,Wang, Fengxue,Yang, Yong,Sun, Na,Ren, Jingqiang,Cheng, Shipeng,Wen, Yongjun,Chen, Xiaoyun,Wang, Hualei.

[14]Genetically modified rabies virus-vectored Ebola virus disease vaccines are safe and induce efficacious immune responses in mice and dogs. Shuai, Lei,Wang, Xijun,Wen, Zhiyuan,Ge, Jinying,Wang, Jinliang,Zhao, Dandan,Bu, Zhigao.

[15]Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Xue, Xianghong,Zheng, Xuexing,Liang, Hongru,Feng, Na,Zhao, Yongkun,Gao, Yuwei,Wang, Hualei,Yang, Songtao,Xia, Xianzhu,Xue, Xianghong,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Xia, Xianzhu. 2014

[16]Aptamers targeting rabies virus-infected cells inhibit street rabies virus in vivo. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gu-Qiu,Li, Ling,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu. 2014

[17]Molecular characterization of China rabies virus vaccine strain. Jiao, Wenqiang,Yin, Xiangping,Li, Zhiyong,Lan, Xi,Li, Xuerui,Tian, Xiaoting,Li, Baoyu,Yang, Bin,Zhang, Yun,Liu, Jixing. 2011

[18]Immunization with recombinant rabies virus expressing Interleukin-18 exhibits enhanced immunogenicity and protection in mice. Gai, Weiwei,Zheng, Wenwen,Song, Yanyan,Gai, Weiwei,Zheng, Xuexing,Wang, Chong,Wong, Gary. 2017

[19]Selection of an aptamer against rabies virus: A new class of molecules with antiviral activity. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gui-Qiu,Xue, Xiang-Hong,Li, Lu,Zheng, Xue-Xing,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu.

[20]Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus. Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Tan, Yeping.

作者其他论文 更多>>

微信小程序