Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus

文献类型: 外文期刊

第一作者: Yang, Yang

作者: Yang, Yang;Qin, Xiaodong;Wang, Guangxiang;Jin, Jiaxin;Shang, Youjun;Zhang, Zhidong;Jin, Jiaxin

作者机构:

关键词: Orf virus;Recombinase polymerase amplification;RPA;Lateral flow;Rapid test;Isothermal nucleic acid amplification

期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )

ISSN: 1743-422X

年卷期: 2016 年 13 卷

页码:

收录情况: SCI

摘要: Background: Orf virus (ORFV) is the causative agent of a severe infectious skin disease (also known as contagious ecthyma) in goats, sheep and other small ruminants. Importantly, ORFV also infect humans which causes a public health concern in the context of changing environment and increase in human populations. The rapid detection is critical in effective control of the disease and urgently needed. Results: A novel "point of care" molecular amplification assay for rapid visual detection of ORFV was developed based on isothermoal recombinase polymerase amplification (RPA) technology in combination with a simpler lateral flow immunoassay strip (ORFV RPA-LFD assay). The developed ORFV RPA-LFD assay was able to detect ORFV in less than 25 min. This assay was highly sensitive, with detection limit of as low as 80 copies/reaction, and highly specific, with no cross-reactions with capripox virus, foot-and-mouth disease virus and peste des petits ruminants virus. Furthermore, the ORFV RPA-LFD assay has good correlation with qPCR assay for detection of ORFV present in clinical samples. Conclusions: The developed ORFV RPA-LFD assay was a sensitive and specific method for rapid detection of ORFV, and has great potential as an onsite molecular diagnostic tool in control of Orf.

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