Cloning and Characterization of a Flavonoid 3'-Hydroxylase Gene from Tea Plant (Camellia sinensis)

文献类型： 外文期刊

第一作者： Zhou, Tian-Shan

作者： Zhou, Tian-Shan;Yu, You-Ben;Xiao, Yao;Li, Dong-Hua;Xiao, Bin;Zhou, Rui;Yu, Oliver;Yang, Ya-Jun

作者机构：

关键词： flavonoid 3-hydroxylase;heterologous expression;flavan 3-ols;nitrogen depletion;Camellia sinensis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2016 年 17 卷 2 期

页码：

收录情况： SCI

摘要： Tea leaves contain abundant flavan-3-ols, which include dihydroxylated and trihydroxylated catechins. Flavonoid 3-hydroxylase (F3H: EC 1.14.13.21) is one of the enzymes in the establishment of the hydroxylation pattern. A gene encoding F3H, designated as CsF3H, was isolated from Camellia sinensis with a homology-based cloning technique and deposited in the GenBank (GenBank ID: KT180309). Bioinformatic analysis revealed that CsF3H was highly homologous with the characterized F3Hs from other plant species. Four conserved cytochrome P450-featured motifs and three F3H-specific conserved motifs were discovered in the protein sequence of CsF3H. Enzymatic analysis of the heterologously expressed CsF3H in yeast demonstrated that tea F3H catalyzed the 3-hydroxylation of naringenin, dihydrokaempferol and kaempferol. Apparent K-m values for these substrates were 17.08, 143.64 and 68.06 M, and their apparent V-max values were 0.98, 0.19 and 0.44 pMmin(-1), respectively. Transcription level of CsF3H in the new shoots, during tea seed germination was measured, along with that of other key genes for flavonoid biosynthesis using real-time PCR technique. The changes in 3,4-flavan-3-ols, 3,4,5-flavan-3-ols and flavan-3-ols, were consistent with the expression level of CsF3H and other related genes in the leaves. In the study of nitrogen supply for the tea plant growth, our results showed the expression level of CsF3H and all other tested genes increased in response to nitrogen depletion after 12 days of treatment, in agreement with a corresponding increase in 3,4-catechins, 3,4,5-catechins and flavan 3-ols content in the leaves. All these results suggest the importance of CsF3H in the biosynthesis of 3,4-catechins, 3,4,5-catechins and flavan 3-ols in tea leaves.

分类号：

  • 相关文献

[1]Molecular cloning, characterization, and heterologous expression of a new kappa-carrageenase gene from marine bacterium Zobellia sp ZM-2. Liu, Zhemin,Mou, Haijin,Li, Guiyang,Mo, Zhaolan. 2013

[2]Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry. Zhao, Liang,Meng, Kun,Bai, Yingguo,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yang, Peilong,Yao, Bin,Zhao, Liang,Song, Wei. 2013

[3]A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress. Zhang, Dayong,He, Xiaolan,Xu, Zhaolong,Xu, Ling,Wei, Peipei,Huang, Yihong,Brestic, Marian,Ma, Hongxiang,Shao, Hongbo,Tong, Jinfeng,Brestic, Marian,Shao, Hongbo. 2016

[4]Reconstruction of the violacein biosynthetic pathway from Duganella sp B2 in different heterologous hosts. Jiang, Pei-xia,Zhang, Chong,Xing, Xin-Hui,Wang, Hai-sheng,Lou, Kai. 2010

[5]Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family. Pearce, Stephen,Dubcovsky, Jorge,Huttly, Alison K.,Prosser, Ian M.,Li, Yi-Dan,Vaughan, Simon P.,Gallova, Barbora,Patil, Archana,Hedden, Peter,Phillips, Andrew L.,Li, Yi-Dan,Coghill, Jane A.,Dubcovsky, Jorge. 2015

[6]Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dismutase and hCuZnSOD in Pichia pastoris. Qu He-zhi,Du shan-shan,Yang Shuo,Huang Lu,Zhang Lei,Xiao Song,Hao Dong-yun,Wang Xiao-ping,Hao Dong-yun. 2010

[7]Immunogenicity of the glycoprotein 5 truncated trans-membrane regions of porcine reproductive and respiratory syndrome virus. Wang, Yinbiao,Xing, Guangxu,Zhang, Gaiping,Wang, Yinbiao,Xing, Guangxu,Guo, Junqing,Qiao, Songlin,Lu, Qingxia,Li, Qingmei,Zhang, Gaiping. 2011

[8]Identification of the streptothricin and tunicamycin biosynthetic gene clusters by genome mining in Streptomyces sp strain fd1-xmd. Yu, Yucong,Tang, Biao,Dai, Ruixue,Zhang, Bo,Chen, Liqiang,Zhao, Guoping,Ding, Xiaoming,Tang, Biao,Yang, Hua,Zhao, Guoping. 2018

[9]Heterologous expression of Helicoverpa armigera cytochrome P450 CYP6B7 in Pichia pastoris and interactions of CYP6B7 with insecticides. Song, Genmiao,Duan, Hongxia,Tang, Tao,Wang, Chen,Qiu, Lihong,Zhao, Chunqing,Tang, Tao.

[10]Rapid cloning, expression and purification of a novel high-activity alkaline phosphatase with detoxification of lipopolysaccharide. Wu, Daichao,Teng, Da,Xi, Di,Wang, Xiumin,Wang, Xiaojie,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua,Wu, Daichao,Dai, Hua,Wu, Daichao,Teng, Da,Xi, Di,Wang, Xiumin,Wang, Xiaojie,Mao, Ruoyu,Zhang, Yong,Dai, Hua,Wang, Jianhua. 2014

[11]Cloning and Functional Characterization of a Fatty Acyl-Acyl Carrier Protein Thioesterase Gene (BnFatB) in Brassica napus L.. Tan, X. L.,Huang, Q.,Tan, R. K.,Zhang, Zh. Y.,Wang, Zh.,Li, X. F.,Wu, L.,Lu, Ch. M.. 2015

[12]Expression Profiles of the Trehalose-6-Phosphate Synthase Gene Associated With Thermal Stress in Ostrinia furnacalis (Lepidoptera: Crambidae). Ge, Feng,Jin, Tingting,Gao, Yulin,He, Kanglai. 2018

[13]Identification and Heterologous Expression of a Delta 4-Fatty Acid Desaturase Gene from Isochrysis sphaerica. Guo, Bing,Jiang, Mulan,Wan, Xia,Gong, Yangmin,Liang, Zhuo,Hu, Chuaniong,Jiang, Mulan,Hu, Chuaniong. 2013

[14]Multiple copies of the target gene enhances plectasin secretion in Pichia pastoris X-33. Teng, Da,Xi, Di,Zhang, Jun,Wang, Xiumin,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua,Teng, Da,Xi, Di,Zhang, Jun,Wang, Xiumin,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua. 2015

[15]Characterization of three novel thermophilic xylanases from Humicola insolens Y1 with application potentials in the brewing industry. Du, Yanlong,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yao, Bin,Du, Yanlong,Zhang, Xiu.

[16]Acyl-CoA oxidase 1 is involved in gamma-decalactone release from peach (Prunus persica) fruit. Zhang, Liping,Li, Haiyan,Gao, Ling,Qi, Yujie,Fu, Wanyi,Li, Xiongwei,Zhou, Xiang,Gao, Zhongshan,Jia, Huijuan,Li, Xiongwei,Gao, Qikang.

[17]Gene cloning, expression, and biochemical characterization of an alkali-tolerant beta-mannanase from Humicola insolens Y1. Luo, Huiying,Wang, Kun,Huang, Huoqing,Shi, Pengjun,Yang, Peilong,Yao, Bin. 2012

[18]Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis. Chen, Hao,Zhang, Yong-Jun,Wu, Kong-Ming,Liang, Ge-Mei,Guo, Yu-Yuan,Liu, Ze-Wen. 2010

[19]A novel thermophilic xylanase from Achaetomium sp Xz-8 with high catalytic efficiency and application potentials in the brewing and other industries. Zhao, Liang,Meng, Kun,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Huang, Huoqing,Wang, Yaru,Yang, Peilong,Yao, Bin,Zhao, Liang. 2013

[20]Two acidic, thermophilic GH28 polygalacturonases from Talaromyces leycettanus JCM 12802 with application potentials for grape juice clarification. Li, Yeqing,Xu, Bo,Li, Yeqing,Wang, Yuan,Tu, Tao,Zhang, Duoduo,Ma, Rui,You, Shuai,Wang, Xiaoyu,Yao, Bin,Luo, Huiying. 2017

作者其他论文 更多>>

微信小程序