A Point Mutation in an F-Box Domain-Containing Protein Is Responsible for Brown Hull Phenotype in Rice

文献类型： 外文期刊

第一作者： Xu Xia

作者： Xu Xia;Zhang Xiao-bo;Shi Yong-feng;Wang Hui-mei;Feng Bao-hua;Li Xiao-hong;Huang Qi-na;Song Li-xin;Guo Dan;He Yan;Wu Jian-li

作者机构：

关键词： Oryza sativa L.;brown hull mutant;OsFBX310 gene;flavonoid metabolism

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2016 年 23 卷 1 期

页码：

收录情况： SCI

摘要： The accumulation of pigments affects the color of rice hulls while only limited information is known about its underlying mechanisms. In the present study, a rice brown hull 6 (bh6) mutant was isolated from an ethane methyl sulfonate (EMS)-induced IR64 mutant bank. Brown pigments started to accumulate in bh6 rice hulls after heading and reached a higher level in mature seeds. Some major agronomic traits including panicle length and 1000-grain weight in bh6 were significantly lower than those in its corresponding wild type IR64, while other agronomic traits such as plant height, growth duration and seed-setting rate were largely similar between the two genotypes. The analysis of pigment content showed that the contents of total flavonoids and anthocyanin in bh6 hulls were significantly higher than those in IR64 hulls. Our results showed that the brown hull phenotype in bh6 was controlled by a single recessive gene which locates on the long arm of chromosome 9. Sequencing analysis detected a single base substitution (G/A) at position 1013 of the candidate gene (LOC_Os09g12150) encoding an F-box domain-containing protein (FBX310). Functional complementation experiment using the wild type allele can rescue the phenotype in bh6. Thus, we named this mutated gene as OsFBX310bh6, an allele of OsFBX310 functioning as an inhibitor of brown hull. The isolation of OsFBX310bh6 and its wild type allele can provide useful experimental materials and will facilitate the studies on revealing the mechanisms of flavonoid metabolism in monocot plants.

分类号：

  • 相关文献

[1]Crop production, nitrogen recovery and water use efficiency in rice-wheat rotation as affected by non-flooded mulching cultivation (NFMC). Ai, YW,Zhang, FS,Lu, SH,Zeng, XZ,Fan, MS. 2005

[2]Heritable alteration in DNA methylation induced by nitrogen-deficiency stress accompanies enhanced tolerance by progenies to the stress in rice (Oryza sativa L.). Von Wettstein, D.,Kou, H. P.,Li, Y.,Song, X. X.,Ou, X. F.,Liu, B.,Kou, H. P.,Li, Y.,Song, X. X.,Ou, X. F.,Liu, B.,Kou, H. P.,Ma, J.,Xing, S. C.. 2011

[3]A simplified genomic DNA extraction protocol for pre-germination genotyping in rice. Duan, Y. B.,Zhao, F. L.,Chen, H. D.,Sheng, W.,Teng, J. T.,Zhang, A. M.,Xue, J. P.,Li, H.,Ni, D. H.,Wei, P. C.. 2015

[4]Inheritance of resistance to rice stripe virus in rice line 'BL 1'. Ise, K,Ishikawa, K,Li, CY,Ye, CR. 2002

[5]Genetic dissection of QTL against phosphate deficiency in the hybrid rice 'Xieyou9308'. Zhang, Yingxin,Anis, Galal Bakr,Wu, Weiming,Yu, Ning,Shen, Xihong,Zhan, Xiaodeng,Cheng, Shihua,Cao, Liyong,Wang, Ruci,Zhang, Yingxin,Wu, Weiming,Shen, Xihong,Zhan, Xiaodeng,Cheng, Shihua,Cao, Liyong,Anis, Galal Bakr. 2018

[6]Characterization and cloning of SMALL GRAIN 4, a novel DWARF11 allele that affects brassinosteroid biosynthesis in rice. Shi, Zhenyuan,Rao, Yuchun,Xu, Jie,Hu, Shikai,Fang, Yunxia,Yu, Haiping,Pan, Jiangjie,Liu, Ruifang,Ren, Deyong,Wang, Xiaohu,Zhu, Yangzhou,Zhu, Li,Dong, Guojun,Zhang, Guangheng,Zeng, Dali,Guo, Longbiao,Hu, Jiang,Qian, Qian,Rao, Yuchun,Zhu, Yangzhou,Xu, Jie. 2015

[7]Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Shi, ZhenYing,Wang, Jiang,Wan, XinShan,Shen, GeZhi,Wang, XinQi,Zhang, JingLiu. 2007

[8]Cytoplasm and cytoplasm-nucleus interactions affect agronomic traits in japonica rice. Tao, DY,Hu, FY,Yang, JY,Yang, GF,Yang, YQ,Xu, P,Li, J,Ye, CR,Dai, LY. 2004

[9]Density alteration of nutrient elements in rice grains of a low phytate mutant. Ren, Xue-Liang,Liu, Qing-Long,Fu, Hao-Wei,Wu, Dian-xing,Shu, Qing-Yao. 2007

[10]Analysis of QTLs for panicle exsertion and its relationship with yield and yield-related traits in rice (Oryza sativa L.). Zhao, C. F.,Chen, T.,Zhao, Q. Y.,Zhou, L. H.,Zhao, L.,Zhang, Y. D.,Zhu, Z.,Yao, S.,Wang, C. L.. 2016

[11]Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice. Chen, Tao,Zhang, Yadong,Zhao, Ling,Zhu, Zhen,Lin, Jing,Zhang, Suobing,Wang, Cailin. 2009

[12]An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.). Duan, Yongbo,Li, Hao,Li, Juan,Ni, Dahu,Song, Fengshun,Li, Li,Yang, Jianbo,Duan, Yongbo,Song, Fengshun,Zhai, Chenguang,Mei, Wenqian,Gui, Huaping,Zhang, Wanggen,Li, Hao,Li, Juan,Ni, Dahu. 2012

[13]Identification and Expression of Genes Involved in Race-specific Blast Resistance in Rice. Hu, Hai-Yan,Zheng, Kang-Le,Zhuang, Jie-Yun,Zheng, Kang-Le,Zhuang, Jie-Yun,Chai, Rong-Yao,Li, Yu-Chuan. 2010

[14]Mapping of a major resistance gene to the brown planthopper in the rice cultivar Rathu Heenati. Sun, LH,Su, CC,Wang, CM,Zhai, HQ,Wan, JM. 2005

[15]Inheritance and mapping of male sterility restoration gene in upland japonica restorer lines. Tao, DY,Xu, P,Li, J,Hu, FY,Yang, YQ,Zhou, JW,Tan, XL,Jones, MP. 2004

[16]Characterisation of magnesium nutrition and interaction of magnesium and potassium in rice. Ding, Y.,Luo, W.,Xu, G.. 2006

[17]Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice. Lin, Chunjing,Hu, Lanjuan,Yang, Jingjing,Zhou, Tianqi,Xu, Chunming,Liu, Bao,Lin, Chunjing,Lin, Xiuyun,Long, Likun,Xing, Shaochen,Dong, Yingshan,Qi, Bao. 2012

[18]Identification of QTLs for cold tolerance at seedling stage in rice (Oryza sativa L.) using two distinct methods of cold treatment. Zhang, Shaohong,Liu, Bin,Zhao, Junliang,Wang, Xiaofei,Yang, Tifeng,Huang, Zhanghui,Zheng, Jingsheng,Peng, Shaobing,Leung, Hei. 2014

[19]Molecular cloning and function analysis of the stay green gene in rice. Jiang, Huawu,Li, Meiru,Liang, Naiting,Yan, Hongbo,Wei, Yubo,Xu, Xinlan,Liu, Jian,Xu, Zhifang,Chen, Fan,Wu, Guojiang.

[20]Clustered QTL for source leaf size and yield traits in rice (Oryza sativa L.). Wang, Peng,Zhou, Guilin,Cui, Kehui,Yu, Sibin,Wang, Peng,Zhou, Guilin,Cui, Kehui,Li, Zhikang,Yu, Sibin,Li, Zhikang.

作者其他论文 更多>>

微信小程序