EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

文献类型： 外文期刊

第一作者： Zhang, Caiyun

作者： Zhang, Caiyun;Shi, Wenshuo;Ma, Keshi;Zhang, Feixiong;Li, Hongjie

作者机构：

关键词： Ca2+ deprivation;EGTA;genomic DNA damage;DNA methylation;RAPD;CRED-RA;Triticum aestivum L.

期刊名称：ACTA SOCIETATIS BOTANICORUM POLONIAE （ 影响因子：0.943； 五年影响因子：1.124 ）

ISSN： 0001-6977

年卷期： 2016 年 85 卷 3 期

页码：

收录情况： SCI

摘要： In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly down-regulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca2+ chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.

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