Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus

文献类型： 外文期刊

第一作者： Yang, Yang

作者： Yang, Yang;Qin, Xiaodong;Wang, Guangxiang;Zhang, Yuen;Shang, Youjun;Zhang, Zhidong;Zhang, Yuen

作者机构：

关键词： Recombinase polymerase amplification assay;RPA assay;Orf virus;Small ruminants

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2015 年 12 卷

页码：

收录情况： SCI

摘要： Background: Orf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. The rapid detection of ORFV is of great importance in disease control and highly needed. A isothermal molecular diagnostic approach, termed recombinase polymerase amplification (RPA), is considered as an novel and rapid alternative techonology to PCR assay. Results: In the present study, a novel fluorescent probe based on RPA assay (ORFV exo RPA assay) was developed. The developed ORFV exo RPA assay was capable of as low as 100 copies of ORFV DNA /reaction and was highly specific, with no cross-reaction with closely related viruses (capripox virus, foot-and-mouth disease virus or peste des petits ruminants virus). Further assessment with clinical samples showed that the developed ORFV exo RPA assay has good correlation with qPCR assays for detection of ORFV. Conclusions: These results suggest that the developed ORFV exo RPA assay is suitable for rapid detection of ORFV.

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