DNA methylation Landscape of body size variation in sheep

文献类型： 外文期刊

第一作者： Cao, Jiaxue

作者： Cao, Jiaxue;Wei, Caihong;Wang, Huihua;Wu, Mingming;Zhang, Li;Zhao, Fuping;Liu, Ruizao;Zhang, Shifang;Du, Lixin;Cao, Jiaxue;Li, Li;Zhong, Tao;Wang, Linjie;Zhang, Hongping;Liu, Dongming;Xie, Zhiyuan;Du, Yongfei;Capellini, Terence D.;Lu, Jian

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2015 年 5 卷

页码：

收录情况： SCI

摘要： Sub-populations of Chinese Mongolian sheep exhibit significant variance in body mass. In the present study, we sequenced the whole genome DNA methylation in these breeds to detect whether DNA methylation plays a role in determining the body mass of sheep by Methylated DNA immunoprecipitation - sequencing method. A high quality methylation map of Chinese Mongolian sheep was obtained in this study. We identified 399 different methylated regions located in 93 human orthologs, which were previously reported as body size related genes in human genome-wide association studies. We tested three regions in LTBP1, and DNA methylation of two CpG sites showed significant correlation with its RNA expression. Additionally, a particular set of differentially methylated windows enriched in the "development process" (GO: 0032502) was identified as potential candidates for association with body mass variation. Next, we validated small part of these windows in 5 genes; DNA methylation of SMAD1, TSC1 and AKT1 showed significant difference across breeds, and six CpG were significantly correlated with RNA expression. Interestingly, two CpG sites showed significant correlation with TSC1 protein expression. This study provides a thorough understanding of body size variation in sheep from an epigenetic perspective.

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