Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments

文献类型： 外文期刊

第一作者： Niu, Xiaoping

作者： Niu, Xiaoping;Qi, Jianmin;Zhang, Gaoyang;Xu, Jiantang;Tao, Aifen;Fang, Pingping;Zhang, Gaoyang;Su, Jianguang

作者机构：

关键词： jute (Corchorus capsularis);qRT-PCR;reference genes;gene expression;abiotic and biotic stress

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： To accurately measure gene expression using quantitative reverse transcription PCR (qRT-PCR), reliable reference gene(s) are required for data normalization. Corchorus capsular's, an annual herbaceous fiber crop with predominant biodegradability and renewability, has not been investigated for the stability of reference genes with qRT-PCR. In this study, 11 candidate reference genes were selected and their expression levels were assessed using qRT-PCR. To account for the influence of experimental approach and tissue type, 22 different jute samples were selected from abiotic and biotic stress conditions as well as three different tissue types. The stability of the candidate reference genes was evaluated using geNorm, NormFinder, and BestKeeper programs, and the comprehensive rankings of gene stability were generated by aggregate analysis. For the biotic stress and NaCl stress subsets, ACT7 and RAN were suitable as stable reference genes for gene expression normalization. For the PEG stress subset, UBC, and DnaJ were sufficient for accurate normalization. For the tissues subset, four reference genes TUB beta, UBI, EF1 alpha, and RAN were sufficient for accurate normalization. The selected genes were further validated by comparing expression profiles of WRKY15 in various samples, and two stable reference genes were recommended for accurate normalization of qRT-PCR data. Our results provide researchers with appropriate reference genes for qRT-PCR in C. capsularis, and will facilitate gene expression study under these conditions.

分类号：

  • 相关文献

[1]Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum. Chen, Xue,Mao, Yingji,Huang, Shengwei,Ni, Jun,Lu, Weili,Hou, Jinyan,Wang, Muting,Zhao, Weiwei,Li, Minghao,Wu, Lifang,Chen, Xue,Mao, Yingji,Huang, Shengwei,Ni, Jun,Lu, Weili,Hou, Jinyan,Wang, Muting,Zhao, Weiwei,Li, Minghao,Wu, Lifang,Chen, Xue,Mao, Yingji,Lu, Weili,Wang, Muting,Lu, Weili,Wang, Muting,Wang, Qiaojian. 2017

[2]Evaluation and validation of reference genes for SYBR Green qRT-PCR normalization in Sesamia inferens (Lepidoptera: Noctuidae). Lu, Yanhui,Zheng, Xusong,Liang, Qi,Xu, Hongxing,Yang, Yajun,Tian, Junce,Lu, Zhongxian,He, Xiaochan. 2015

[3]Evaluation of appropriate reference genes for gene expression studies in pepper by quantitative real-time PCR. Bin, Wang Shu,Wei, Liu Ke,Ping, Diao Wei,Li, Zhi,Wei, Ge,Bing, Liu Jin,Gui, Pan Bao,Jian, Wan Hong,Feng, Chen Jin,Wei, Liu Ke.

[4]Reference gene selection for quantitative real-time PCR normalization in different cherry genotypes, developmental stages and organs. Ye, Xia,Zhang, Fangming,Tao, Yonghuan,Song, Shangwei,Fang, Jinbao.

[5]Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR. Fang, Peng,Sun, Feng,Lan, Ying,Du, Linlin,Zhou, Yijun,Zhou, Tong,Fang, Peng,Sun, Feng,Lan, Ying,Du, Linlin,Zhou, Yijun,Zhou, Tong,Fang, Peng,Lu, Rongfei,Shen, Wenbiao. 2015

[6]Evaluation of putative internal reference genes for gene expression normalization in Nannochloropsis sp by quantitative real-time RT-PCR. Cao, Shaona,Zhang, Xiaowen,Ye, Naihao,Fan, Xiao,Xu, Dong,Cao, Shaona,Wang, Yitao,Wang, Wenqi,Liang, Chengwei. 2012

[7]Validation of Reference Genes for RT-qPCR Studies of Gene Expression in Preharvest and Postharvest Longan Fruits under Different Experimental Conditions. Wu, Jianyang,Zhang, Hongna,Liu, Liqin,Li, Weicai,Wei, Yongzan,Shi, Shengyou. 2016

[8]Candidate Reference Genes Selection and Application for RT-qPCR Analysis in Kenaf with Cytoplasmic Male Sterility Background. Chen, Peng,Khan, Aziz,Chen, Lihong,Liao, Xiaofang,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong,Liu, Dongmei. 2017

[9]Molecular Cloning and Induced Expression of Six Small Heat Shock Proteins Mediating Cold-Hardiness in Harmonia axyridis (Coleoptera: Coccinellidae). Wang, Hui-Juan,Shi, Zuo-Kun,Shen, Qi-Da,Xu, Cai-Di,Wang, Shi-Gui,Tang, Bin,Wang, Bing,Wang, Su,Meng, Zhao-Jun. 2017

[10]Transcript Profiling of Hevea brasiliensis during Latex Flow. Tian, Wei-Min. 2017

[11]Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze). Hao, Xinyuan,Yang, Yajun,Xiao, Bin,Hao, Xinyuan,Horvath, David P.,Chao, Wun S.,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao. 2014

[12]Inhibitory effect and mode of action of chitosan solution against rice bacterial brown stripe pathogen Acidovorax avenae subsp avenae RS-1. Yang, Chunlan,Li, Bin,Ge, Mengyu,Zhou, Kaile,Xie, Guanlin,Wang, Yanli,Sun, Guochang,Luo, Ju,Ibrahim, Muhammad. 2014

[13]Isolation, structural analysis, and expression characteristics of the maize TIFY gene family. Zhang, Zhongbao,Li, Xianglong,Han, Meng,Wu, Zhongyi,Zhang, Zhongbao,Li, Xianglong,Han, Meng,Wu, Zhongyi,Yu, Rong,Han, Meng,Wu, Zhongyi.

[14]X1-homologous genes family as central components in biotic and abiotic stresses response in maize (Zea mays L.). Zhang, Zhongbao,Chen, Yajuan,Zhao, Dan,Li, Ruifen,Wang, Hongzhi,Zhang, Jiewei,Wei, Jianhua,Zhang, Zhongbao,Chen, Yajuan,Zhao, Dan,Li, Ruifen,Wang, Hongzhi,Zhang, Jiewei,Wei, Jianhua.

[15]Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress. Tian Qing-song,Du Jian-cai,Han Bing,Wang Shu-yan,Wu Zhi-juan,Li Xiao-quan,Han Bing. 2016

[16]Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation. Wang, Jiying,Wang, Yanping,Wang, Huaizhong,Hao, Xiaojing,Wu, Ying,Guo, Jianfeng. 2014

[17]Identification of reference genes for reverse transcription quantitative real-time PCR normalization in pepper (Capsicum annuum L.). Wan, Hongjian,Yuan, Wei,Ruan, Meiying,Ye, Qingjing,Wang, Rongqing,Li, Zhimiao,Zhou, Guozhi,Yao, Zhuping,Zhao, Jing,Liu, Shujun,Yang, Yuejian. 2011

[18]Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus). Yang, Chang Geng,Tian, Juan,Liu, Wei,Wu, Fan,Jiang, Ming,Wen, Hua,Wang, Xian Li.

[19]The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza. Zhang, Xiaowen,Xu, Dong,Ye, Naihao,Dong, Meitao,Wang, Wenqi,Chi, Xiaoyuan,Mou, Shanli,Xu, Jianfang.

[20]Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms. Wang, Hou-Ling,Li, Lan,Yuan, Chao,Tian, Qianqian,Su, Yanyan,Li, Hui-Guang,Zhao, Lin,Yin, Weilun,Zhao, Rui,Xia, Xinli,Wang, Hou-Ling,Yin, Weilun,Tang, Sha. 2015

作者其他论文 更多>>

微信小程序