Identification of Ramie Genes in Response to Pratylenchus coffeae Infection Challenge by Digital Gene Expression Analysis
文献类型: 外文期刊
第一作者: Yu, Yongting
作者: Yu, Yongting;Zeng, Liangbin;Yan, Zhun;Liu, Touming;Sun, Kai;Zhu, Taotao;Zhu, Aiguo
作者机构:
关键词: Boehmeria nivea (L;) Gaudich;root lesion nematode;digital gene expression;resistance related genes;cystatin
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2015 年 16 卷 9 期
页码:
收录情况: SCI
摘要: Root lesion disease, caused by Pratylenchus coffeae, seriously impairs the growth and yield of ramie, an important natural fiber crop. The ramie defense mechanism against P. coffeae infection is poorly understood, which hinders efforts to improve resistance via breeding programs. In this study, the transcriptome of the resistant ramie cultivar Qingdaye was characterized using Illumina sequence technology. About 46.3 million clean pair end (PE) reads were generated and assembled into 40,826 unigenes with a mean length of 830 bp. Digital gene expression (DGE) analysis was performed on both the control roots (CK) and P. coffeae-challenged roots (CH), and the differentially expressed genes (DEGs) were identified. Approximately 10.16 and 8.07 million cDNA reads in the CK and CH cDNA libraries were sequenced, respectively. A total of 137 genes exhibited different transcript abundances between the two libraries. Among them, the expressions of 117 and 20 DEGs were up- and down-regulated in P. coffeae-challenged ramie, respectively. The expression patterns of 15 candidate genes determined by qRT-PCR confirmed the results of DGE analysis. Time-course expression profiles of eight defense-related genes in susceptible and resistant ramie cultivars were different after P. coffeae inoculation. The differential expression of protease inhibitors, pathogenesis-related proteins (PRs), and transcription factors in resistant and susceptible ramie during P. coffeae infection indicated that cystatin likely plays an important role in nematode resistance.
分类号:
- 相关文献
作者其他论文 更多>>
-
Multiple omics revealed the growth-promoting mechanism of Bacillus velezensis strains on ramie
作者:Wang, Xin;Wang, Yanzhou;Fu, Yafen;Zhai, Yang;Bai, Xuehua;Liu, Tongying;Li, Guang;Zeng, Liangbin;Zhu, Siyuan
关键词:plant growth-promoting rhizobacteria; microbial diversity; bacterial community; metabolomics correlation; ramie
-
Lipids signaling and unsaturation of fatty acids participate in ramie response to submergence stress and hypoxia-responsive gene regulation
作者:Shao, Deyi;Yu, Chunming;Chen, Yu;Qiu, Xiaojun;Chen, Jikang;Zhao, Haohan;Chen, Kunmei;Wang, Xiaofei;Chen, Ping;Gao, Gang;Zhu, Aiguo
关键词:Ramie; Submergence stress; Lipid metabolism
-
Genome-Wide Methylation Landscape Uncovers the Role of DNA Methylation in Ramie (Boehmeria nivea L.) Bast Fiber Growth
作者:Li, Fu;Luo, Bingbing;Peng, Qingzhong;Yi, Langbo;Li, Fu;Gao, Song;Liu, Touming;Li, Fu;Wang, Yanzhou;Rao, Jing
关键词:ramie; DNA methylation; fiber growth; differential expression; NAC gene
-
Adaptive responses and metabolic strategies of Novosphingobium sp. ES2-1-17β-estradiol analyzed through integration of genomic and proteomic approaches
作者:Li, Shunyao;Yang, Wei;Mo, Jingjing;Wang, Yubing;Li, Yucheng;Gao, Yanzheng;Sun, Kai;Lu, Chao
关键词:Microbial degradation; Steroid estrogens; Novosphingobium; Molecular mechanisms; Genomics and proteomics
-
Genomic variation and candidate genes dissect quality and yield traits in Boehmeria nivea (L.) Gaudich
作者:Shi, Yaliang;Huang, Kunyong;Chen, Jikang;Niu, Juan;Sun, Zhimin;Yu, Na;Qiu, Xiaojun;Shi, Yingying;Chen, Jing;Zhong, Yicheng;Wang, Yue;Chen, Jianhua;Luan, Mingbao;Zhu, Aiguo;Shi, Yaliang;Huda, Md. Nurul
关键词:Ramie; Cellulose fiber; Yield trait; Quantitative trait loci; Candidate gene
-
Phytoremediation and environmental effects of three Amaranthaceae plants in contaminated soil under intercropping systems
作者:Huang, Rong;Xing, Chen;Yang, Yuanru;Yu, Wang;Zeng, Liangbin;Tan, Zhijian;Li, Yanqiong;Li, Zhian
关键词:Phytoextraction; Intercropping; Monoculture; Cadmium; Soil microbes
-
Study on remediation of cadmium contaminated paddy field by ramie (Boehmeria nivea L.) floating island and its supporting technology
作者:Zhao, Yijia;Yu, Chunming;Chen, Ping;Mou, Pan;Chen, Jikang;Gao, Gang;Wang, Xiaofei;Zhu, Aiguo;Chen, Kunmei;Chen, Kunmei
关键词:Cadmium contaminated paddy field; Hydroponic ramie ( Boehmeria nivea L.); Phytoremediation; Floating island technology; Remediation model