Immunoglobulin genomics in the prairie vole (Microtus ochrogaster)

文献类型： 外文期刊

第一作者： Qin, Tong

作者： Qin, Tong;Zhu, Huabin;Wang, Dong;Du, Weihua;Hao, Haisheng;Zhao, Huijing

作者机构：

关键词： Prairie vole;lmmunoglobulin heavy chain;lmmunoglobulin light chain

期刊名称：IMMUNOLOGY LETTERS （ 影响因子：3.685； 五年影响因子：3.54 ）

ISSN： 0165-2478

年卷期： 2015 年 166 卷 2 期

页码：

收录情况： SCI

摘要： In science, the prairie voles are ideal models for studying the regulatory mechanisms of social behavior in humans. The utility of the prairie vole as a biology model can be further enhanced by characterization of the genes encoding components of the immune system. Here, we report the genomic organization of the prairie vole immunoglobulin heavy and light chain genes. The prairie vole IgH locus on chromosome 1 spans over 1600 kb, and consists of at least 79 V-H segments (28 potentially functional genes, 2 ORFs and 49 pseudogenes), 7 D-H segments, 4 J(H) segments, four constant region genes ( mu, gamma, epsilon, and alpha), and two transmembrane regions of 8 gene. The Ig kappa locus, found on three scaffolds (JH996430, JH996605 and JH996566), contains a totle of 124 V-kappa segments (47 potentially functional genes, 1 ORF and 76 pseudogenes), 5J(kappa) segments and a single C-kappa gene. Two different transcriptional orientations were determined for these V-kappa gene segments. In contrast, the Igk locus on scaffold JH996473 and JH996489 includes 21 V-lambda gene segments (14 potentially functional genes, 1 ORF and 6 pseudogenes), all with the same transcriptional polarity as the downstream J(lambda)-C-lambda cluster. Phylogenetic analysis and sequence alignments suggested the prairie vole's large germline VH, V-kappa and V-lambda gene segments appear to form limited gene families. Therefore, this species may generate antibody diversity via a gene conversion-like mechanism associated with its pseudogene reserves. (C) 2015 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

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