Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants

文献类型： 外文期刊

第一作者： Chen, Mingna

作者： Chen, Mingna;Yang, Qingli;Wang, Tong;Chen, Na;Pan, Lijuan;Chi, Xiaoyuan;Yang, Zhen;Wang, Mian;Yu, Shanlin;Chi, Xiaoyuan

作者机构：

关键词： Arachis hypogaea L.;Agrobacterium-mediated transformation;cotyledon and mesocotyl;sonication;phytohormones;plantlet recovery

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2015 年 95 卷 4 期

页码：

收录情况： SCI

摘要： Four genotypes of peanut and two sources of explants (cotyledon and mesocotyl) were tested for their susceptibility to genetic transformation by the Agrobacterium tumefaciens strain LBA4404 that harbored the binary vector pCAMBIA1301. This plasmid contains the hygromycin phosphotransferase (hpt) and beta-glucuronidase (GUS) genes, each under the control of a CaMV35S promoter. Comparative analyses of regeneration and transformation efficiencies indicated that mesocotyl was a better target tissue than cotyledon, and peanut genotypes of that mature early and have relatively small seeds (such as Huayu 26 and Huayu 20) were shown to be comparatively responsive to transformation. Sonication of explants soaked with solutions containing Agrobacterium was shown to optimize transformation. Culture of explants on medium supplemented with 3 mg L-1 indole-3-butyric acid and 0.1 mg L-1 napthaleneacetic acid enabled vigorous rooting from almost all transgenic shoots. More than 85% of the transplanted plants could produce morphologically normal flowers and pods with viable seeds. Phenotypic and genotypic monitoring of the inheritance of hpt and GUS genes through two generations indicated the expected 3:1 inheritance. Our results make Agrobacterium-mediated transformation a viable and useful tool for both breeding and functional genomic analysis of peanut.

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