Transcription Analysis of the Beta-Glucosidase Precursor in Wild-Type and l-4i Mutant Bombyx mori (Lepidoptera: Bombycidae)

文献类型： 外文期刊

第一作者： Kang, Lequn

作者： Kang, Lequn;Wu, Fan;Zhao, Qiaoling;Huang, Fei;Zhao, Qiaoling

作者机构：

关键词： beta-glucosidase precursor;BmpreBG;lethal mutant

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2015 年 15 卷

页码：

收录情况： SCI

摘要： Lethal fourth-instar larvae (l-4i) mutant of Bombyx mori, a recently discovered novel mutant, die from energy depletion due to genetic mutation. Beta-glucosidase is a common digestive enzyme that hydrolyzes cellulose in the diet to provide energy. In this study, the mRNA expression profiles of B. mori beta-glucosidase precursor (BmpreBG) were characterized by reverse transcription polymerase chain reaction and quantitative real-time polymerase chain reaction. The transcription level of BmpreBG varied in different tissues and developmental stages, except in the pupa and moth, which are the no-diet period. Remarkably, the mRNA expression level of BmpreBG was sharply reduced in l-4i but not in the wild type, which suggested that the digestive function of the mutant was severely damaged. This was consistent with the l-4i phenotypic traits of not eating mulberries, lack of energy, and ultimate death. 50-rapid amplification of cDNA ends showed, for the first time, that BmpreBG has a 160-bp 50-untranslated region. These findings suggested that B. mori beta-glucosidase precursor was involved in the death process of l-4i mutant larvae.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序