Simultaneous detection of novel H7N9 and other influenza A viruses in poultry by multiplex real-time RT-PCR
文献类型: 外文期刊
第一作者: Xu, Xiaolong
作者: Xu, Xiaolong;Bao, Hongmei;Ma, Yong;Sun, Jiashan;Zhao, Yuhui;Wang, Yunhe;Shi, Jianzhong;Zeng, Xianying;Li, Yanbing;Wang, Xiurong;Chen, Hualan
作者机构:
关键词: Multiplex rRT-PCR;H7N9 virus;Influenza A virus
期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )
ISSN: 1743-422X
年卷期: 2015 年 12 卷
页码:
收录情况: SCI
摘要: Background: A novel reassortant H7N9 influenza A virus has crossed the species barrier from poultry to cause human infections in China in 2013 and 2014. Rapid detection of the novel H7N9 virus is important to detect this virus in poultry and reduce the risk of an epidemic in birds or humans. Findings: In this study, a multiplex real-time RT-PCR (rRT-PCR) assay for rapid detection of H7N9 and other influenza A viruses was developed. To evaluate the assay, various influenza A viruses, other avian respiratory viruses, and 1,070 samples from poultry were tested. Fluorescence signals corresponding to H7 and N9 subtypes were detected only when H7 and N9 subtypes were present, while the fluorescence signal for the influenza A M gene was detected in all specimens with influenza A strains. The fluorescent signal can be detected in dilutions as low as 56 copies per reaction for the H7, N9 and M genes. Intra-and inter-assay variability tests showed a reliable assay. In poultry samples, a comparison of rRT-PCR with virus isolation showed a high level of agreement. Conclusions: The multiplex rRT-PCR assay in this study has good specificity, sensitivity and reproducibility, and will be useful for laboratory surveillance and rapid diagnosis of H7N9 and other influenza A viruses.
分类号:
- 相关文献
作者其他论文 更多>>
-
Advances in Isorhamnetin Treatment of Malignant Tumors: Mechanisms and Applications
作者:Mei, Chen;Liu, Ying;Jiang, Zhaoling;Liu, Zhenyi;Zhi, Yan;Wang, Hongjun;Lyu, Xueze;Lyu, Xueze;Xu, Xiaolong
关键词:isorhamnetin; malignant tumor; antitumor mechanism; clinical application; natural medicine
-
Enzymatic Characterization of a Rumen Microorganism-Derived Multifunctional Glycoside Hydrolase and Its GH26 Domain with Mannanase Activity
作者:Zhao, Xin;Ma, Yong;Wang, Zhihong;Wu, Hao;Zhang, Hong;Gao, Li;Wen, Tong;Guo, Fangfang
关键词:
Segatella bryantii ; endo-beta-1,4-glucanase; endo-beta-1,4-mannanase; multifunctional enzyme -
The study of epidermal wax of different apple varieties and functional analysis of MdMYB94 regulating wax biosynthesis
作者:Liu, Shasha;Huo, Wenping;Wang, Zhongkang;Xu, Xiaolong;Chen, Yizhou;Shang, Yimeng;Hou, Hongmin;Zhang, Yugang;Jiang, Shenghui;Liu, Shasha;Huo, Wenping;Wang, Zhongkang;Xu, Xiaolong;Chen, Yizhou;Shang, Yimeng;Hou, Hongmin;Zhang, Yugang;Jiang, Shenghui;Chen, Min
关键词:Apple; Fruit development; Cuticular wax; Transcriptional regulation
-
Cellular SLC35B4 promotes internalization during influenza A virus entry
作者:Wang, Guangwen;Jiang, Li;Yan, Ya;Kong, Fandi;Li, Qibing;Zhang, Jie;Hou, Shuangshuang;Wang, Bo;Wang, Xiurong;Kong, Huihui;Deng, Guohua;Shi, Jianzhong;Tian, Guobin;Zeng, Xianying;Chen, Hualan;Li, Chengjun
关键词:influenza A virus; internalization; AGRN; heparan sulfate modification
-
A Strand-Specific Quantitative RT-PCR Method for Detecting vRNA, cRNA, and mRNA of H7N9 Avian Influenza Virus in a Mouse Model
作者:Wang, Bo;Wang, Guangwen;Wang, Yi-han;Liu, Xuwei;Li, Manman;Kong, Huihui;Chen, Hualan;Jiang, Li;Li, Chengjun
关键词:avian influenza virus; qRT-PCR; replication kinetics
-
N6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins
作者:Wang, Qian;Xu, Shuai;Shen, Wentao;Wei, Yanli;Han, Lu;Wang, Zhengxiang;Yu, Yingying;Liu, Minxuan;Liu, Junwen;Zhu, Qiyun;Xu, Shuai;Zhu, Qiyun;Deng, Guohua;Chen, Hualan
关键词:influenza Avirus; N6-methyladenosine; viral RNA; viral ribonucleoprotein complex; replication
-
KRT6A Restricts Influenza A Virus Replication by Inhibiting the Nuclear Import and Assembly of Viral Ribonucleoprotein Complex
作者:Chang, Yu;Shan, Zhibo;Li, Qibing;Wang, Yihan;Wang, Bo;Wang, Guangwen;Chen, Hualan;Jiang, Li;Li, Chengjun;Shi, Wenjun
关键词:influenza A virus; KRT6A; vRNP complex; NP; nuclear import; assembly