Reducing microbial ureolytic activity in the rumen by immunization against urease therein

文献类型： 外文期刊

第一作者： Zhao, Shengguo

作者： Zhao, Shengguo;Wang, Jiaqi;Zheng, Nan;Zhao, Shengguo;Wang, Jiaqi;Zheng, Nan;Bu, Dengpan;Sun, Peng;Yu, Zhongtang

作者机构：

关键词： Immunization;Rumen;Urease;Ureolytic activity

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2015 年 11 卷

页码：

收录情况： SCI

摘要： Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein. Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria. Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

分类号：

  • 相关文献

[1]Impacts of the transgenic CrylAc and CpTI insect-resistant cotton SGK321 on selected soil enzyme activities in the rhizosphere. Xie, M.,Li, C. Y.,Wu, G.,Peng, D. L.. 2014

[2]Enantioselective effects of metalaxyl on soil enzyme activity. Yue, Heng,Fang, Song,Zhang, Yizhi,Ning, Yang,Yu, Weisong,Kong, Fanyu,Qiu, Jun,Yue, Heng,Fang, Song,Zhang, Yizhi,Ning, Yang,Yu, Weisong,Kong, Fanyu,Qiu, Jun.

[3]Ureolytic microbial community is modulated by fertilization regimes and particle-size fractions in a Black soil of Northeastern China. Wang, Li,Luo, Xuesong,Liao, Hao,Chen, Wen,Cai, Peng,Huang, Qiaoyun,Wang, Li,Luo, Xuesong,Cai, Peng,Huang, Qiaoyun,Wei, Dan. 2018

[4]Immunization with Toxoplasma gondii GRA17 Deletion Mutant Induces Partial Protection and Survival in Challenged Mice. Wang, Jin-Lei,Zhu, Wei-Ning,Chen, Kai,Li, Ting-Ting,Yue, Dong-Mei,Zhang, Xiao-Xuan,Huang, Si-Yang,Zhu, Xing-Quan,Elsheikha, Hany M.,Zhu, Wei-Ning. 2017

[5]EXPRESSION OF THE BARLEY YELLOW DWARF VIRUS-GAV MOVEMENT PROTEIN AND ITS DETECTION IN THE INFECTED AND TRANSGENIC PLANTS. Wang, X.,Liu, Y.,Peng, Y.,Zhou, G..

[6]Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast. Sun, M,Qian, KX,Su, N,Chang, HY,Liu, JX,Chen, GF.

[7]Characterization of Schistosoma japonicum estrogen-related receptor beta like 1 and immunogenicity analysis of the recombinant protein. Wu, Xiujuan,Zhao, Bin,Hong, Yang,Li, Xuezheng,Peng, Jinbiao,Zhang, Juan,Wang, Fei,Shi, Yaojun,Fu, Zhiqiang,Lin, Jiaojiao.

[8]Immunogenicity and protective efficacy in monkeys of purified inactivated Vero-cell SARS vaccine. Qin, E,Shi, HY,Tang, L,Wang, C,Chang, GH,Ding, ZF,Zhao, K,Wang, J,Chen, Z,Yu, M,Si, BY,Liu, JY,Wu, DL,Cheng, XJ,Yang, BA,Peng, WM,Meng, QW,Liu, BH,Han, WG,Yin, XA,Duan, HY,Zhan, DW,Tian, L,Li, SL,Wu, JS,Tan, G,Li, Y,Li, YC,Liu, YG,Liu, H,Lv, FS,Zhang, Y,Kong, XG,Fan, BC,Jiang, T,Xu, SL,Wang, XM,Li, CW,Wu, XH,Deng, YQ,Zhao, M,Zhu, QY.

[9]Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Li, Guo-Xin,Tian, Zhi-Jun,Yu, Hai,Jin, Yuan-Yuan,Hou, Shao-Hua,Zhou, Yan-Jun,Liu, Tian-Qiang,Hu, Shou-Ping,Tong, Guang-Zhi,Li, Guo-Xin,Tong, Guang-Zhi,Li, Guo-Xin,Yu, Hai,Zhou, Yan-Jun,Tong, Guang-Zhi.

[10]Immunization of mice by Hollow Mesoporous Silica Nanoparticles as carriers of Porcine Circovirus Type 2 ORF2 Protein. Sun, Shi-Qi. 2012

[11]Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Li, GJ,Chen, WZ,Yan, WY,Zhao, K,Liu, MQ,Zhang, J,Fei, L,Xu, QX,Sheng, ZT,Lu, YG,Zheng, ZX.

[12]H9 subtype influenza vaccine in MDCK single-cell suspension culture with stable expression of TMPRSS2: Generation and efficacy evaluation. Feng, Lei,Tang, Yinghua,Wu, Peipei,Chu, Xuan,Wang, Weifeng,Hou, Jibo,Feng, Lei,Tang, Yinghua,Wu, Peipei,Hou, Jibo.

[13]Studies on enhancing embryo quantity and quality by immunization against inhibin in repeatedly superovulated Holstein heifers and the associated endocrine mechanisms. Liu, Y. P.,Chen, R. A.,Mao, X. B.,Wei, Y. M.,Yu, J. N.,Li, H.,Shi, Z. D..

[14]Combined DNA vaccines formulated either in DDA or in saline protect cattle from Mycobacterium bovis infection. Cai, H,Tian, X,Hu, XD,Li, SX,Yu, DH,Zhu, YX.

[15]Effect of immunization against GnRH on hypothalamic and testicular function in rams. Han, Xingfa,Gu, Longjun,Cao, Xiaohan,Du, Xiaogang,Zeng, Xianyin,Xia, Chenyang,Feng, Jing,Song, Tianzeng.

[16]Immunization against inhibin improves in vivo and in vitro embryo production. Yan, Leyan,Li, Hui,Shi, Zhendan.

[17]Biochemical and kinetic characterization of the multifunctional beta-glucosidase/beta-xylosidase/alpha-arabinosidase, Bgxa1. Gruninger, R. J.,Gong, X.,Forster, R. J.,McAllister, T. A.,Gong, X.. 2014

[18]Isolation and biochemical characterization of two lipases from a metagenomic library of China Holstein cow rumen. Liu, Kailang,Wang, Jiaqi,Bu, Dengpan,Zhao, Shengguo,Yu, Ping,Li, Dan,McSweeney, Chris. 2009

[19]Feruloyl and acetyl esterase production of an anaerobic rumen fungus Neocallimastix sp YQ2 effected by glucose and soluble nitrogen supplementations and its potential in the hydrolysis of fibrous feedstuffs. Yang, H. J.,Yue, Q.,Yang, H. J.,Cao, Y. Ch.,Zhang, D. F.,Jiang, Y. H.,Wang, J. Q.. 2009

[20]Rumen Bacterial Diversity of Water Buffalo (Bubalus bubalis) as Influenced by Concentrate Levels. Yang, Chengjian,Zou, Caixia,Liang, Xin,Wei, Shengju,Li, Shulu,Liang, Xianwei,Yang, Bingzhuang,Luo, Hua. 2013

作者其他论文 更多>>

微信小程序