Rapid On-Site Sensing Aflatoxin B-1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay

文献类型： 外文期刊

第一作者： Zhang, Zhaowei

作者： Zhang, Zhaowei;Tang, Xiaoqian;Wang, Du;Zhang, Qi;Li, Peiwu;Ding, Xiaoxia;Zhang, Zhaowei;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Wang, Du;Zhang, Qi;Li, Peiwu;Ding, Xiaoxia;Ding, Xiaoxia

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Aflatoxin B-1 poses grave threats to food and feed safety due to its strong carcinogenesis and toxicity, thus requiring ultrasensitive rapid on-site determination. Herein, a portable immunosensor based on chromatographic time-resolved fluoroimmunoassay was developed for sensitive and on-site determination of aflatoxin B-1 in food and feed samples. Chromatographic time-resolved fluoroimmunoassay offered a magnified positive signal and low signal-to-noise ratio in time-resolved mode due to the absence of noise interference caused by excitation light sources. Compared with the immunosensing performance in previous studies, this platform demonstrated a wider dynamic range of 0.2-60 mu g/kg, lower limit of detection from 0.06 to 0.12 mu g/kg, and considerable recovery from 80.5% to 116.7% for different food and feed sample matrices. It was found to be little cross-reactivity with other aflatoxins (B-2, G(1), G(2), and M-1). In the case of determination of aflatoxin B-1 in peanuts, corn, soy sauce, vegetable oil, and mouse feed, excellent agreement was found when compared with aflatoxin B-1 determination via the conversational high-performance liquid chromatography method. The chromatographic time-resolved fluoroimmunoassay affords a powerful alternative for rapid on-site determination of aflatoxin B1 and holds a promise for food safety in consideration of practical food safety and environmental monitoring.

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