Peptidoglycan Recognition Protein S2 From Silkworm Integument: Characterization, Microbe-Induced Expression, and Involvement in the Immune-Deficiency Pathway

文献类型: 外文期刊

第一作者: Yang, Jie

作者: Yang, Jie;Wang, Xiaonan;Wu, Jinmei;Tang, Shunming;Shen, Zhongyuan;Wu, Jinmei

作者机构:

关键词: Bombyx mori;innate immunity;peptidoglycan recognition protein;RNA interference

期刊名称:JOURNAL OF INSECT SCIENCE ( 影响因子:1.857; 五年影响因子:1.904 )

ISSN: 1536-2442

年卷期: 2015 年 15 卷

页码:

收录情况: SCI

摘要: Peptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori. A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP (DmPGRP-SA). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli-and Bacillus subtilis-challenged B. mori was measured using semiquantitative reverse transcriptase polymerase chain reaction analysis. The expression of BmPGRP-S2 was upregulated at 24 h by E. coli and Ba. subtilis challenge. In addition, in the integument of B. mori, RNAi knockdown of BmPGRP-S2 caused an obvious reduction in the transcription expression of the transcription factor Relish and in antibacterial effector genes Attacin, Gloverin, and Moricin. The results indicated that BmPGRP-S2 participates in the signal transduction pathway of B. mori.

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