An effective biphase system accelerates hesperidinase-catalyzed conversion of rutin to isoquercitrin

文献类型： 外文期刊

第一作者： Wang, Jun

作者： Wang, Jun;Gong, An;Yang, Cai-Feng;Bao, Qi;Shi, Xin-Yi;Han, Bei-Bei;Wu, Fu-An;Wang, Jun;Gong, An;Yang, Cai-Feng;Bao, Qi;Shi, Xin-Yi;Han, Bei-Bei;Wu, Fu-An;Wang, Jun;Wu, Xiang-Yang

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2015 年 5 卷

页码：

收录情况： SCI

摘要： Isoquercitrin is a rare, natural ingredient with several biological activities that is a key precursor for the synthesis of enzymatically modified isoquercitrin (EMIQ). The enzymatic production of isoquercitrin from rutin catalyzed by hesperidinase is feasible; however, the bioprocess is hindered by low substrate concentration and a long reaction time. Thus, a novel biphase system consisting of [Bmim][BF4]: glycine-sodium hydroxide (pH 9) (10:90, v/v) and glyceryl triacetate (1:1, v/v) was initially established for isoquercitrin production. The biotransformation product was identified using liquid chromatography-mass spectrometry, and the bonding mechanism of the enzyme and substrate was inferred using circular dichroism spectra and kinetic parameters. The highest rutin conversion of 99.5% and isoquercitrin yield of 93.9% were obtained after 3 h. The reaction route is environmentally benign and mild, and the biphase system could be reused. The substrate concentration was increased 2.6-fold, the reaction time was reduced to three tenths the original time. The three-dimensional structure of hesperidinase was changed in the biphase system, which a-helix and random content were reduced and beta-sheet content was increased. Thus, the developed biphase system can effectively strengthen the hesperidinase-catalyzed synthesis of isoquercitrin with high yield.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序