文献类型： 外文期刊
第一作者： Gao, Yuhua
作者： Gao, Yuhua;Li, Xiangchen;Guan, Weijun;Ma, Yuehui;Gao, Yuhua;Li, Xiangchen;Zheng, Dong
作者机构：
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2015 年 16 卷 3 期
页码：
收录情况： SCI
摘要： We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).
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