Quantitative Trait Loci Mapping for Plant Height in Tobacco using Linkage and Association Mapping Methods

文献类型： 外文期刊

第一作者： Cheng, Lirui

作者： Cheng, Lirui;Yang, Aiguo;Jiang, Caihong;Ren, Min;Zhang, Yu;Feng, Quanfu;Wang, Shaomei;Luo, Chenggang;Guan, Yongsheng

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期刊名称：CROP SCIENCE （ 影响因子：2.319； 五年影响因子：2.631 ）

ISSN： 0011-183X

年卷期： 2015 年 55 卷 2 期

页码：

收录情况： SCI

摘要： Plant height (PH) is one of the most important agronomic traits in tobacco (Nicotiana tabacum L.). To gain a better understanding of its genetic basis, the F-2 and F-2:3 populations derived from NC82 (P-1) and Kang88 (P-2) cross were developed. The 193 F-2 individuals and 145 F-2:3 lines were further used to identify quantitative trait loci (QTL) affecting to PH in tobacco. Two main quantitative trait loci (M-QTL), designated qPH-6 and qPH-12, were mapped on genetic linkage groups 6 and 12, respectively, using linkage mapping (LM). The QTL qPH-12 was identified as stably expressed in different tobacco generations as well as in various environments. To further verify the chromosomal interval harboring qPH-12, a total of 96 accessions were subsequently evaluated for PH at two different sites and genotyped by 46 markers evenly distributed on chromosome 12. We found a total of four significant marker-trait associations that were identified with three different SSR markers (P < 0.01) and could explain 11.7 to 14.8% of the phenotypic variation in PH. One marker, PT55174, showed a significant association with PH (P < 0.01) and explained 13.9 and 14.4% of the phenotypic variation at two different sites. Taken together, our results could provide a new avenue for investigating the QTL in tobacco by combining LM and association mapping (AM), and facilitating our understanding of the inheritance of PH for marker-assisted selection (MAS) in tobacco.

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