Cloning, expression and antiviral activity of mink alpha-interferons

文献类型: 外文期刊

第一作者: Zhang, Hai-ling

作者: Zhang, Hai-ling;Zhao, Jian-jun;Chai, Xiu-li;Zhang, Lei;Bai, Xue;Hu, Bo;Liu, Hao;Yan, Xi-jun;Zhang, Dong-liang;Ye, Ming;Wu, Wei

作者机构:

关键词: Mink;Interferon subtype;Antiviral activity

期刊名称:BMC VETERINARY RESEARCH ( 影响因子:2.741; 五年影响因子:2.955 )

ISSN: 1746-6148

年卷期: 2015 年 11 卷

页码:

收录情况: SCI

摘要: Background: As a key link between innate and adaptive immune responses, the interferon (IFN) system is the first line of defense against viral infection. IFN, and in particular, IFN-alpha, has been used clinically as an effective therapeutic agent for viral infections. However, different subtypes of IFN-alpha demonstrate distinct antiviral activity. Therefore, it is important to identify IFN-alpha subtypes with high antiviral activity for the development of genetically engineered antiviral drugs. Results: In this study, we cloned the genes for 13 IFN-alpha subtypes from peripheral blood lymphocytes of the mink. The homologies of the 13 mink IFN-alpha genes were 93.6-99.3% and 88.8-98.4% at the nucleotide and amino acid sequence levels, respectively. In contrast to human and canine IFN-alpha subtypes, most mink IFN-alpha subtypes contained two N-glycosylation sites. We expressed and purified 13 mink IFN-alpha subtypes in Escherichia coli. The cytopathic effect inhibition assay showed that all the 13 recombinant mink IFN-alpha subtypes inhibited the propagation of vesicular stomatitis virus in WISH cells, with IFN-alpha 2 and IFN-alpha 12 demonstrating the highest activities. Furthermore, recombinant mink IFN-alpha 2 and IFN-alpha 12 significantly suppressed the propagation of canine distemper virus in Vero cells, with IFN-alpha 2 demonstrating the highest activity. Conclusions: We identified the mink IFN-alpha 2 subtype as a promising candidate for the development of effective antiviral drugs.

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