Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

文献类型: 外文期刊

第一作者: Suo, Huayi

作者: Suo, Huayi;Xie, Jie;Song, Jia-Le;Song, Jia-Le;Yi, Ruokun;Zhou, Yalin;Zhu, Kai;Zhao, Xin;Liu, Zhenhu;Zhao, Xin

作者机构:

关键词: polysaccharide;Larimichthys crocea swim bladder;HCT-116 human colon cancer cells;apoptosis;gene

期刊名称:ONCOLOGY LETTERS ( 影响因子:2.967; 五年影响因子:2.575 )

ISSN: 1792-1074

年卷期: 2015 年 9 卷 2 期

页码:

收录情况: SCI

摘要: Larimichthys crocea swim bladder is a traditional food and medicine widely used in China. The in vitro anticancer effects of polysaccharide of L. crocea swim bladder (PLCSB) in HCT-116 human colon cancer cells was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 mu g/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration-dependent manner. In particular, 400 mu g/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6-diamidino-2-phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT-116 cancer cells, the expression of apoptosis and metastasis-associated genes was analyzed by reverse transcription-polymerase chain reaction and western blot analysis. A total of 400 mu g/ml PLCSB significantly induced apoptosis in HCT-116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase-3, -8, and -9, as well as Fas and the downregulation of B-cell lymphoma 2 (Bcl-2), Bcl-extra large and Fos ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT-116 colon cancer cells, in vitro.

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