Construction and characterization of a bacterial artificial chromosome library for the allotetraploid Gossypium tomentosum

文献类型： 外文期刊

第一作者： F. Liu

作者： F. Liu;Y.H. Wang;H.Y. Gao;C.Y. Wang;Z.L. Zhou;X.Y. Cai;X.X. Wang;Z.S. Zhang;K.B. Wang

作者机构：

关键词： Wild cotton;Gossypium tomentosum;Drought tolerance;Bacterial artificial chromosome library;Verticillium wilt resistance;SSR marker

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Gossypium tomentosum is a wild allotetraploid species with the (AD)(5) genome. It is characterized by many useful traits including finer fiber fineness, drought tolerance, and Fusarium and Verticillium resistance. We constructed the first bacterial artificial chromosome library for Gossypium tomentosum. With high quality and broad coverage, this library includes 200,832 clones, with an average insert size of about 122 kb and fewer than 3% empty clones. Our library is approximately 10-fold the size of the (AD)(5)-genome (2400 Mb) and provides a 99.7% probability of isolating genes of interest or their sequences. Seven of eight simple sequence repeats markers that are located on five different chromosomes and linked with resistance to Verticillium wilt could amplify the 50 superpools and obtained one to five hits. This high capacity library will be an important genomic resource for classifying and analyzing the evolution of allotetraploid cotton species as well as for isolating disease-resistance and drought-tolerance genes.

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