Analysis of alcohol dehydrogenase inhibitors from Desmodium styracifolium using centrifugal ultrafiltration coupled with HPLC-MS
文献类型： 外文期刊
第一作者： Liu, Liangliang
作者： Liu, Liangliang;Chen, Miao;Chen, Xiaoqing;Chen, Xiaoqing
作者机构：
关键词： alcohol dehydrogenases;aromadendrin;centrifugal ultrafiltration;Desmodium styracifolium;formononetin
期刊名称：JOURNAL OF THE SERBIAN CHEMICAL SOCIETY （ 影响因子：1.24； 五年影响因子：1.144 ）
ISSN： 0352-5139
年卷期： 2015 年 80 卷 8 期
页码：
收录情况： SCI
摘要： Alcohol dehydrogenase (ADH) inhibitors play an important role in the treatment of human methanol or ethylene glycol poisoning and the suppression of acetaldehyde accumulation in alcoholics. In this study, centrifugal ultrafiltration coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was utilized to screen and identify ADH inhibitors from an ethyl acetate extract of Desmodium styracifolium (Osb.) Merr. The experimental conditions of the centrifugal ultrafiltration were optimized. Under the optimum conditions (ADH concentration: 37.5 mu g mL(-1), incubation time: 90 min, pH 7.0 and temperature: 15 degrees C), formononetin and aromadendrin were successfully screened and identified from the ethyl acetate extract of D. styracifolium. The screening result was verified by ADH inhibition assays. The IC50 values of formononetin and aromadendrin were 70.8 and 84.7 mu g mL(-1), which were in accordance with their degrees of binding. Aromadendrin was for the first time reported to have inhibitory activity on ADH. This method provides an effective way to screen active compounds from natural products.
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