High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from 'Candidatus Liberibacter asiaticus'

文献类型： 外文期刊

第一作者： Wang Xue-feng

作者： Wang Xue-feng;Chen Jiao-yue;Tan Jin;Duan Suo;Zhou Chang-yong;Chen Jiao-yue;Duan Suo;Deng Xiao-ling;Chen Jian-chi

作者机构：

关键词： Huanglongbing;'Candidatus Liberibacter asiaticus';miniature inverted-repeat transposable element

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from 'Candidatus Liberibacter asiaticus' known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immediate upstream gene of the two MITEs was predicted to be a putative transposase. The goal of this study is to analyze the sequence variation in the upstream putative transposase of MITEs and explore the possible correlation between sequence variation of transposase gene and MITE activity. PCR and sequence analysis showed that 12 sequence types were found in six major amplicon types from 43 representative 'Ca. L. asiaticus' isolates from China, the United States and Brazil. Out of the 12 sequence types, three (14, T5-2, T6) were reported for the first time. Recombination events were found in the two unique sequence types (T5-2 and 16) which were detected in all Brazilian isolates. Notably, no sequence variation or recombination events were detected in the upstream putative transposase gene of MCLas-A, suggesting the conservation of the transposase gene might be closely related with the MITE activity. Phylogenetic analysis demonstrated two well supported clades including five subclades were identified, clearly reflecting the geographical origins of isolates, especially that of Ruili isolates, Sao Paulo isolates and a few Florida isolates.

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