High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants
文献类型: 外文期刊
第一作者: Shen Wan-xia
作者: Shen Wan-xia;Zhou Chang-yong;Shen Wan-xia;Smith, Neil A.;Wang Ming-bo;Shen Wan-xia;Zhou Chang-yong
作者机构:
关键词: Nicotiana benthamiana;transformation;satellite RNA;RNA silencing;small RNA
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2014 年 13 卷 5 期
页码:
收录情况: SCI
摘要: RNA silencing is a fundamental plant defence and gene control mechanism in plants that are directed by 20-24 nucleotide (nt) small interfering RNA (siRNA) and microRNA (miRNA). Infection of plants with viral pathogens or transformation of plants with RNA interference (RNAi) constructs is usually associated with high levels of exogenous siRNAs, but it is unclear if these siRNAs interfere with endogenous small RNA pathways and hence affect plant development. Here we provide evidence that viral satellite RNA (satRNA) infection does not affect siRNA and miRNA biogenesis or plant growth despite the extremely high level of satRNA-derived siRNAs. We generated transgenic Nicotiana benthamiana plants that no longer develop the specific yellowing symptoms generally associated with infection by Cucumber mosaic virus (CMV) Y-satellite RNA (Y-Sat). We then used these plants to show that CMV Y-Sat infection did not cause any visible phenotypic changes in comparison to uninfected plants, despite the presence of high-level Y-Sat siRNAs. Furthermore, we showed that the accumulation of hairpin RNA (hpRNA)-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not significantly affected by CMV Y-Sat infection. Taken together, our results suggest that the high levels of exogenous siRNAs associated with viral infection or RNAi-inducing transgenes do not saturate the endogenous RNA silencing machineries and have no significant impact on normal plant development.
分类号:
- 相关文献
作者其他论文 更多>>
-
Decreasing detection frequency of MITE (MCLas-A) in the population of 'Candidatus Liberibacter asiaticus' recently collected in southern China
作者:Cui Xue-jin;Zeng Chun-hua;Liu Ke-hong;Teng Cal-ling;Zhou Chang-yong;Wang Xue-feng
关键词:Huanglongbing; 'Candidatus Liberibacter asiaticus'; miniature inverted-repeat transposable element; MITE
-
Limited infection by 'Candidatus Liberibacter asiaticus' in 'Valencia' sweet orange trees in the presence of Citrus tristeza virus
作者:Fu Shi-min;Zhou Chang-yong;Hartung, John S.;Gouin, Cristina
关键词:huanglongbing; plant defense; plant protection
-
Development of a sensitive and reliable droplet digital PCR assay for the detection of 'Candidatus Liberibacter asiaticus'
作者:Zhong Xi;Liu Xue-lu;Zhou Chang-yong;Wang Xue-feng;Lou Bing-hai
关键词:citrus Huanglongbing;early diagnosis;droplet digital PCR
-
Small RNA deep sequencing reveals full-length genome of Citrus yellow vein clearing virus in Chongqing, China
作者:Yu Yun-qi;Wu Qiong;Yu Yun-qi;Wu Qiong;Wang Xue-feng;Cao Meng-ji;Zhou Chang-yong;Su Hua-nan
关键词:citrus;Citrus yellow vein clearing virus (CYVCV);small RNA deep sequencing
-
Complete genome sequence analysis of two Citrus tatter leaf virus (CTLV) isolates from China
作者:Song Zhen;Li Zhong-an;Liu Ke-hong;Zhou Chang-yong
关键词:Citrus tatter leaf virus;Apple stem grooving virus;phylogenetic analysis;genome
-
The complete genome sequence of Citrus vein enation virus from China
作者:Huang Ai-jun;Song Zhen;Cao Meng-ji;Chen Hong-ming;Li Zhong-an;Zhou Chang-yong;Huang Ai-jun
关键词:Citrus vein enation virus (CVEV);genome sequence;Enamovirus
-
High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from 'Candidatus Liberibacter asiaticus'
作者:Wang Xue-feng;Chen Jiao-yue;Tan Jin;Duan Suo;Zhou Chang-yong;Chen Jiao-yue;Duan Suo;Deng Xiao-ling;Chen Jian-chi
关键词:Huanglongbing;'Candidatus Liberibacter asiaticus';miniature inverted-repeat transposable element