Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots

文献类型： 外文期刊

第一作者： Li, W. C.

作者： Li, W. C.;Ji, S. D.;Wang, X. C.;Li, Z. K.;Zhang, H. C.;Tian, C. Z.;Liu, Y. L.;Duan, C. X.

作者机构：

关键词： Ion beam;Transformation;Rice;Proteinase

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Corn DNA was introduced into dry seeds of rice (cv. 'YuJing-6') by ion beam irradiation. Proteinase activities in rice seedling roots were subsequently analyzed by renaturation electrophoresis at pH 4.5, 7.0, and 8.5. Proteinase activity was more pronounced on gels at higher pH. Irradiation of rice seedling roots caused the loss of some proteinase bands at all pH conditions although a novel 50-kDa band was found at both pH 7.0 and 8.5. No new proteinase activity was detected at pH 4.5. However, novel bands and bands showing stronger activity were observed at pH 7.0 and 8.5. The data indicate that the expression of proteinases in rice seedling roots was altered following low energy ion beam mediated transformation with corn DNA.

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