Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum
文献类型: 外文期刊
第一作者: Fan Jia
作者: Fan Jia;Sun Jing-rui;Chen Ju-lian
作者机构:
关键词: Schizaphis graminum;salivary protein C002;cDNA clone;siRNA
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2015 年 14 卷 4 期
页码:
收录情况: SCI
摘要: The full-length cDNA of functionally-unknown salivary protein 0002 in Schizaphis graminum was cloned using rapid amplification of cDNA ends (RACE) and designated as SgC002 (GenBank accession no. KC977563). It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 kDa and a predicted cleavage site of N-terminal signal peptide between the 24th and the 25th residues. SgC002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing SgC002-specific 476-siRNA, but not 546-siRNA to aphids through artificial diet significantly suppressed SgC002 expression. Silencing SgC002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.
分类号:
- 相关文献
作者其他论文 更多>>
-
Population genetic structure of Sitobion miscanthi in China
作者:Sun Jing-xuan;Li Qian;Tan Xiao-ling;Fan Jia;Zhang Yong;Qin Yao-guo;Chen Ju-lian;Sun Jing-xuan;Francis, Frederic;Sun Jing-xuan
关键词:Sitobion miscanthi; molecular marker; COI; symbiotic bacteria; phylogenetic tree
-
Screening and evaluation for antibiosis resistance of the spring wheat accessions to the grain aphid, Sitobion miscanthi (Takahashi) (Hemiptera: Aphididae)
作者:Gebretsadik, Kifle Gebreegziabiher;Zhang Yong;Chen Ju-lian;Gebretsadik, Kifle Gebreegziabiher
关键词:Sitobion miscanthi; spring wheat accessions; antibiosis resistance; electronic penetration graph; high-performance liquid chromatography; phenolic acid concentrations
-
Effect of the Secondary Symbiont Hamiltonella defensa on Fitness and Relative Abundance of Buchnera aphidicola of Wheat Aphid, Sitobion miscanthi
作者:Fan Jia;Sun Jingxuan;Chen Julian;Li Qian;Wang Manqun
关键词:Sitobion miscanthi; symbionts; Hamiltonella defensa; insect fitness; Buchnera aphidicola; relative abundance
-
Influence of Garlic Intercropping or Active Emitted Volatiles in Releasers on Aphid and Related Beneficial in Wheat Fields in China
作者:Chen Ju-lian;Sun Jing-rui;Cheng Deng-fa;Zhou Hai-bo;Francis, Frederic;Haubruge, Eric;Liu Yong;Bragard, Claude
关键词:wheat;garlic;intercropping;semiochemical release;Sitobion avenae;natural enemies
-
Genetic Variation Among the Geographic Population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China Inferred from Mitochondrial COI Gene Sequence
作者:Xu Zhao-huan;Chen Ju-lian;Cheng Deng-fa;Xu Zhao-huan;Liu Yong;Francis, Frederic
关键词:Sitobion avenae;insect pest;mitochondrial DNA;mtDNA-COI gene;geographic variation