Highly sensitive colorimetric detection of 17 beta-estradiol using split DNA aptamers immobilized on unmodified gold nanoparticles

文献类型： 外文期刊

第一作者： Liu, Jinchuan

作者： Liu, Jinchuan;Bai, Wenhui;Niu, Shucao;Zhu, Chao;Yang, Shuming;Chen, Ailiang;Liu, Jinchuan;Bai, Wenhui;Niu, Shucao;Zhu, Chao;Yang, Shuming;Chen, Ailiang

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2014 年 4 卷

页码：

收录情况： SCI

摘要： Gold nanoparticle (AuNP) based colorimetric aptasensor have been developed for many analytes recently largely because of the ease of detection, high sensitivity, and potential for high-throughput analysis. Most of the target aptamers for detection have short sequences. However, the approach shows poor performance in terms of detection sensitivity for most of the long-sequence aptamers. To address this problem, for the first time, we split the 76 mer aptamer of 17 beta-estradiol into two short pieces to improve the AuNP based colorimetric sensitivity. Our results showed that the split P1 + P2 still retained the original 76 mer aptamer's affinity and specificity but increased the detection limit by 10-fold, demonstrating that as low as 0.1 ng/mL 17 beta-estradiol could be detected. The increased sensitivity may be caused by lower aptamer adsorption concentration and a lower affinity to the AuNPs of a short single-strand DNA (ssDNA) sequence. Our study provided a new way to use long-sequence aptamers to develop a highly sensitive AuNP-based colorimetric aptasensor.

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