Anti-Idiotypic Nanobody-Phage Based Real-Time Immuno-PCR for Detection of Hepatocarcinogen Aflatoxin in Grains and Feedstuffs

文献类型： 外文期刊

第一作者： Lei, Jiawen

作者： Lei, Jiawen;Li, Peiwu;Zhang, Qi;Wang, Yanru;Zhang, Zhaowei;Ding, Xiaoxia;Zang, Wen;Lei, Jiawen;Li, Peiwu;Wang, Yanru;Zhang, Zhaowei;Ding, Xiaoxia;Zang, Wen;Li, Peiwu;Zhang, Qi;Zhang, Zhaowei;Lei, Jiawen;Li, Peiwu;Zhang, Qi;Wang, Yanru;Zhang, Zhaowei;Ding, Xiaoxia;Li, Peiwu;Ding, Xiaoxia;Zang, Wen

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2014 年 86 卷 21 期

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收录情况： SCI

摘要： Aflatoxins are a group of extremely toxic small molecules that have been involved in human hepatic and extrahepatic carcinogenesis as causative agents. Herein, we developed a real-time immuno polymerase chain reaction (IPCR) assay for the accurately quantitative detection of aflatoxins in agri-products base on a M13 phage containing aflatoxin anti-idiotypic nanobody and its encoding DNA which was used to design the specific primers. The limit of detection (LOD) of the assay is 0.02 ng/mL, which exhibits a 4-fold improvement over traditional phage ELISA. The developed method was successfully validated with the samples of corn, rice, peanut, and feedstuff, which are major aflatoxin-contaminated agri-products. And the recoveries were from 77.05 to 122.16%. For further validation, the developed assay was also compared with a reference HPLC method for the analysis of aflatoxins in corn and peanuts, and concordant results (R-2 = 0.991) were obtained. In this context, this study provides a novel opportunity to analyze aflatoxins in agri-products.

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