Identification of differential gene expression profiles between winter dormant and sprouting axillary buds in tea plant (Camellia sinensis) by suppression subtractive hybridization

文献类型： 外文期刊

第一作者： Wang, Xinchao

作者： Wang, Xinchao;Hao, Xinyuan;Ma, Chunlei;Cao, Hongli;Yue, Chuan;Wang, Lu;Zeng, Jianming;Yang, Yajun;Wang, Xinchao;Hao, Xinyuan;Ma, Chunlei;Cao, Hongli;Yue, Chuan;Wang, Lu;Zeng, Jianming;Yang, Yajun;Hao, Xinyuan

作者机构：

关键词： Tea plant (C. sinensis);Axillary bud dormancy;Differential gene expression;Suppression subtractive hybridization (SSH)

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2014 年 10 卷 5 期

页码：

收录情况： SCI

摘要： Tea plant (Camellia sinensis (L.) O. Kuntze) is an important cash crop. In temperate regions, bud dormancy in winter and budbreak in spring are important biological phenomena for the tea plant life cycle. To understand the molecular mechanism of dormancy maintenance and release in tea plant, the differentially expressed genes in dormant and sprouting axillary buds were investigated in two cultivars (special early-sprouting tea cultivar 'Camellia sinensis cv. Longjing 43' ('LJ') and late-sprouting tea cultivar 'C. sinensis cv. Zhenghe Dabaicha' ('ZD')), using suppression subtractive hybridization (SSH) approach. Four high performance complementary DNA (cDNA)-SSH libraries ('LJ' sprouting bud library, 'LJ' dormant bud library, 'ZD' sprouting bud library and 'ZD' dormant bud library) were constructed, and 1,736 valid ESTs were obtained, in which 1,242 ESTs were unique to the sprouting bud libraries and 494 ESTs were unique to the dormant bud libraries. Based on sequence matching and gene ontology analysis, 1,287 unigenes consisting of 208 contigs and 1,079 singletons were identified, in which 995 had Blast hits. The putative functions of differentially regulated sequences were involved in most aspects of plant biological processes. The quality and expression patterns of partial ESTs from these four libraries were validated by qRT-PCR. We identified numerous differentially expressed genes mainly involved in stress response, water metabolism, cell division regulation, energy metabolism and hormone regulation from dormant and sprouting bud libraries. This study provides general information on tea plant axillary bud dormancy and release at the transcriptional level and provides some hypotheses for further exploration on the mechanism of bud dormancy and budbreak in tea plant.

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