Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus

文献类型： 外文期刊

第一作者： Liu, Jinyue

作者： Liu, Jinyue;Jiang, Wenbo;Xia, Yaying;Pang, Yongzhen;Liu, Jinyue;Jiang, Wenbo;Xia, Yaying;Pang, Yongzhen;Liu, Jinyue;Liu, Jinyue;Wang, Xuemin;Pang, Yongzhen;Shen, Guoan

作者机构：

关键词： Genistein;Isoflavonoids;Isoflavonoid prenyl-transferase;Lotus japonicus;Wighteone

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2018 年 59 卷 1 期

页码：

收录情况： SCI

摘要： Prenylated isoflavonoids have been found in several legume plants, and they possess various biological activities that play important roles in both plant defense and human health. However, it is still unknown whether prenylated isoflavonoids are present in the model legume plant Lotus japonicus. In the present study, we found that the prenylated isoflavonoid wighteone was produced in L. japonicus when leaf was supplemented with genistein. Furthermore, a novel prenyltransferase gene, LjG6DT, was identified, which shared high similarity with and was closely related to several known prenyltransferase genes involved in isoflavonoid biosynthesis. The recombinant LjG6DT protein expressed in yeast exhibited prenylation activity toward genistein as an exclusive substrate, which produced wighteone, a prenylated genistein at the C-6 position that occurs normally in legume plants. The LjG6DT-green fluorescent protein (GFP) fusion protein is targeted to plastids. The transcript level of LjG6DT is induced by glutathione, methyl jasmonate and salicylic acid, implying that LjG6DT is involved in stress response. Overexpression of LjG6DT in L. japonicus hairy roots led to increased accumulation of wighteone when genistein was supplied, indicating that LjG6DT is functional in vivo. Feeding assays with the upstream intermediate naringenin revealed that accumulation of wighteone in L. japonicus was dependent on genistein supplementation, and accumulation of wighteone is competed by genistein methylation. This study demonstrated that phytoalexin wighteone is inducibly produced in L. japonicus, and it provides new insight into the biosynthesis and accumulation of prenylated isoflavonoids in legume plants.

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