The deduced role of a chitinase containing two nonsynergistic catalytic domains

文献类型： 外文期刊

第一作者： Zhu, Weixing

作者： Zhu, Weixing;Wang, Jing;Zhou, Yong;Duan, Yanwei;Qu, Mingbo;Yang, Qing;Liu, Tian;Zhu, Weixing;Wang, Jing;Zhou, Yong;Duan, Yanwei;Qu, Mingbo;Yang, Qing;Yang, Qing

作者机构：

关键词： chitin;chitinase;chitin synthesis;chitin degradation;synergy

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY （ 影响因子：7.652； 五年影响因子：4.956 ）

ISSN： 2059-7983

年卷期： 2018 年 74 卷

页码：

收录情况： SCI

摘要： The glycoside hydrolase family 18 chitinases degrade or alter chitin. Multiple catalytic domains in a glycoside hydrolase family 18 chitinase function synergistically during chitin degradation. Here, an insect group III chitinase from the agricultural pest Ostrinia furnacalis (OfChtIII) is revealed to be an arthropod-conserved chitinase that contains two nonsynergistic GH18 domains according to its catalytic properties. Both GH18 domains are active towards single-chained chitin substrates, but are inactive towards insoluble chitin substrates. The crystal structures of each unbound GH18 domain, as well as of GH18 domains complexed with hexa-N-acetyl-chitohexaose or penta-N-acetyl-chitopentaose, suggest that the two GH18 domains possess endo-specific activities. Physiological data indicated that the developmental stage-dependent gene-expression pattern of OfChtIII was the same as that of the chitin synthase OfChsA but significantly different from that of the chitinase OfChtI, which is indispensable for cuticular chitin degradation. Additionally, immunological staining indicated that OfChtIII was co-localized with OfChsA. Thus, OfChtIII is most likely to be involved in the chitin-synthesis pathway.

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