Expression and characterization of a recombinant porcinized antibody against the E2 protein of classical swine fever virus

文献类型： 外文期刊

第一作者： Chen, Shucheng

作者： Chen, Shucheng;Li, Su;Sun, Huimin;Li, Yongfeng;Ji, Shengwei;Song, Kun;Zhang, Lingkai;Luo, Yuzi;Sun, Yuan;Liu, Pinghuang;Qiu, Hua-Ji;Chen, Shucheng;Ma, Jifei

作者机构：

关键词： Classical swine fever virus;Monoclonal antibody;Genetic engineering antibody;Chimeric expression;Suspension culture

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2018 年 102 卷 2 期

页码：

收录情况： SCI

摘要： Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious and economically important disease of pigs. The envelope glycoprotein E2 of CSFV is the major antigen that induces neutralizing antibodies and confers protection against CSFV infections. Previously, we developed a murine monoclonal antibody (MAb), HQ06, against the E2 protein of CSFV. To produce the antibody conveniently and stably, the genes coding for the variable regions of the heavy and light chains of HQ06 and constant region genes from the swine antibody were fused and cloned into lentiviral expression vectors to express a recombinant porcinized MAb (rHQ06(Sw)) in mammalian cells. rHQ06(Sw) was able to react with the E2 protein or the CSFV virions specifically in different assays. Notably, rHQ06(Sw) could neutralize CSFV infection in a dose-dependent manner. Taken together, the functional porcinized MAb rHQ06(Sw) was generated, which can be used to develop novel diagnostic assays or to investigate the structure and functions of the E2 protein.

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