Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest

文献类型： 外文期刊

第一作者： Wu, Kongming

作者： Wu, Kongming;Wang, Guirong;Li, Fengqi;Li, Wei;Lin, Yong-Jun;Pickett, John A.;Birkett, Michael A.;Zhou, Jing-Jiang

作者机构：

关键词： (E)-4;8-dimethyl-1;3;7-nonatriene (DMNT);parasitic wasp;transgenic rice

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.228； 五年影响因子：7.791 ）

ISSN： 0140-7791

年卷期： 2018 年 41 卷 1 期

页码：

收录情况： SCI

摘要： Volatile terpenoids play a key role in plant defence against herbivory by attracting parasitic wasps. We identified seven terpene synthase genes from lima bean, Phaseolus lunatus L. following treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus. Four of the genes (Pltps2, Pltps3, Pltps4 and Pltps5) were up-regulated with their derived proteins phylogenetically clustered in the TPS-g subfamily and PlTPS3 positioned at the base of this cluster. Recombinant PlTPS3 was able to convert geranyl diphosphate and farnesyl diphosphate to linalool and (E)-nerolidol, the latter being precursor of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). Recombinant PlTPS4 showed a different substrate specificity and produced linalool and (E)-nerolidol, as well as (E,E)-geranyllinalool from geranylgeranyl diphosphate. Transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and DMNT than wild-type plants, whereas transgenic rice expressing Pltps4 produced (S)-linalool, DMNT and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT). In laboratory bioassays, female Cotesia chilonis, the natural enemy of the striped rice stemborer, Chilo suppressalis, were significantly attracted to the transgenic plants and their volatiles. We further confirmed this with synthetic blends mimicking natural rice volatile composition. Our study demonstrates that the transformation of rice to produce volatile terpenoids has the potential to enhance plant indirect defence through natural enemy recruitment. Homoterpenes are semiochemicals which modify insect behaviour and which are synthesized by terpene synthases. We identified, by Next generation sequencing (NGS), and functionally characterized, several terpene synthase genes from the lima bean, Phaseolus lunatus, following infestation with either spider mites or the elicitor alamethicin. Two up-regulated genes were transformed into an elite rice variety, enhancing homoterpene production and attraction of the natural enemy of rice pests, the parasitoid wasp Cotesia chilonis. This work demonstrated that the transformation of rice to produce volatile terpenoids has the potential to enhance plant defence through beneficial enemy recruitment.

分类号：

  • 相关文献

[1]Over-expression of (1,3;1,4)--D-glucanase isoenzyme EII gene results in decreased (1,3;1,4)--D-glucan content and increased starch level in barley grains. Han, Ning,Na, Chenglong,Chai, Yuqiong,Chen, Jianshu,Zhang, Zhongbo,Bai, Bin,Bian, Hongwu,Zhu, Muyuan,Zhang, Yuhong.

[2]Molecular characterization and expression profiles of olfactory receptor genes in the parasitic wasp, Microplitis mediator (Hymenoptera: Braconidae). Gu, Shao-Hua,Wang, Shan-Ning,Guo, Yu-Yuan,Zhang, Yong-Jun,Ma, Long,Liu, Ze-Wen,Zhou, Jing-Jiang. 2014

[3]Design, synthesis and fungicidal activity of N-substituted benzoyl-1,2,3,4-tetrahydroquinolyl-1-carboxamide. Xu, Yan,Du, Juan,Yang, Xin-Ling,Xu, Gao-Fei,Ling, Yun,Yuan, Hui-Zhu.

[4]Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.. Xu, Gangming,Li, Yingying,Zheng, Wei,Peng, Xiang,Li, Wen,Yan, Yanchun.

[5]Structural diversity-guided convenient construction of functionalized 4H-1,3,4-oxadiazin-5(6H)-one derivatives. Ke, Shaoyong,Wang, Kaimei,Zhang, Zhigang,Zhang, Ya-Ni,Liang, Ying,Shi, Liqiao,Jiang, Aibing,Yang, Ziwen.

[6]Superoxide radical and auxin are implicated in redistribution of root growth and the expression of auxin and cell-cycle genes in cadmium-stressed rice. Zhao, F. Y.,Hu, F.,Han, M. M.,Liu, W.,Zhang, S. Y..

[7]Design, Synthesis and Bioactivity of Novel Glycosylthiadiazole Derivatives. Zong, Guanghui,Zhao, Hanqing,Jiang, Rui,Zhang, Jianjun,Liang, Xiaomei,Wang, Daoquan,Zhao, Hanqing,Li, Baoju,Shi, Yanxia.

[8]A Study of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Induced Liver Injury in Jian Carp (Cyprinus carpio var. Jian) Using Precision-Cut Liver Slices. Du, Jin-Liang,Cao, Li-Ping,Yin, Guo-Jun,Du, Jin-Liang,Cao, Li-Ping,Yin, Guo-Jun,Liu, Ying-Juan,Jia, Rui,Yin, Guo-Jun.

[9]Influences of nitrification inhibitor 3,4-dimethyl pyrazole phosphate on nitrogen and soil salt-ion leaching. Yu Qiaogang,Ye Xuezhu,Yu Qiaogang,Chen Yingxu,Zhang Zhijian,Tian Guangming.

[10]Simultaneous determination of chlorpyrifos and 3,5,6-trichloro-2-pyridinol in duck muscle by modified QuEChERS coupled to gas chromatography tandem mass spectrometry (GC-MS/MS). Li, Rui,Ji, Xiaofeng,Qian, Mingrong,Zhou, Yu,Wang, Qiang,Li, Rui,Ji, Xiaofeng,Qian, Mingrong,Zhou, Yu,Wang, Qiang,Li, Rui,Ji, Xiaofeng,Qian, Mingrong,Zhou, Yu,Wang, Qiang,Li, Rui,Ji, Xiaofeng,Qian, Mingrong,Zhou, Yu,Wang, Qiang,He, Liang,Zhou, Ting.

[11]proteomic Analysis of Peanut Seed Storage Proteins and Genetic Variation in a Potential Peanut Allergen. Guo, Baozhu,Liang, Xianquiang,Chung, Si-Yin,Maleki, Soheila J.,Holbrook, C. Corley.

[12]Evaluation of nitrification inhibitor 3,4-dimethyl pyrazole phosphate on nitrogen leaching in undisturbed soil columns. Yu, Qiaogang,Chen, Yingxu,Ye, Xuezhu,Zhang, Qiuling,Zhang, Zhijian,Tian, Ping.

[13]Synthesis and antibacterial activity of oxime ester derivatives containing 1,2,4-triazole or 1,3,4-oxadiazole moiety. Wang, Xiaobin,Zhong, Xinmin,Zhu, Xuesong,Li, Qin,Zhang, Juping,Ruan, Xianghui,Xue, Wei,Wang, Hua.

[14]Effects of combined application of organic and inorganic fertilizers plus nitrification inhibitor DMPP on nitrogen runoff loss in vegetable soils. Yu, Qiaogang,Ma, Junwei,Zou, Ping,Lin, Hui,Sun, Wanchun,Yin, Jianzhen,Fu, Jianrong.

[15]Decomposition of 3,5-dinitrobenzamide in aqueous solution during UV/H2O2 and UV/TiO2 oxidation processes. Yan, Yingjie,Wang, Wei,Yuan, Shoujun,Hu, Zhen-Hu,Liao, Qi-Nan,Ji, Feng.

[16]Coordination Frameworks based on 3,5-Bis(imidazole-1-yl)pyridine and Aromatic Dicarboxylate Ligands: Synthesis, Crystal Structures, and Photoluminescent Properties. Xu, Jiakun,Sun, Mi,Xu, Jiakun,Yan, Xincheng,Bi, Caifeng,Sun, Xiaochun,Yang, Lirong.

[17]Identification of two combined genes responsible for dechlorination of 3,5,6-trichloro-2-pyridinol (TCP) in Cupriavidus pauculus P2. Cao, Li,Wu, Guang,Li, Mingxing,Jiang, Jiandong,He, Jian,Li, Shunpeng,Hong, Qing,Xu, Jianhong.

[18]Synthesis, Biological Evaluation, and Structure-Activity Relationship Study of Novel Stilbene Derivatives as Potential Fungicidal Agents. He, Daohang,Jian, Weilin,Liu, Xianping,Shen, Huifang,Song, Shaoyun.

[19]Heterologous expression of GmSIP1;3 from soybean in tobacco showed and growth retardation and tolerance to hydrogen peroxide. Zhang, Dayong,Huang, Yihong,Wan, Qun,Xu, Zhaolong,Shao, Hongbo,Shao, Hongbo,Kumar, Manoj,Pandey, Girdhar K..

[20]Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference. Ma, Jin,Song, Yunzhi,Wu, Bin,Li, Kaidong,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong. 2011

作者其他论文 更多>>

微信小程序