Housefly (Musca domestica) and Blow Fly (Protophormia terraenovae) as Vectors of Bacteria Carrying Colistin Resistance Genes

文献类型： 外文期刊

第一作者： Zhang, Jilei

作者： Zhang, Jilei;Wang, Jiawei;Chen, Li;Yassin, Afrah Kamal;Li, Jing;Wang, Chengming;Yassin, Afrah Kamal;Kelly, Patrick;Butaye, Patrick;Butaye, Patrick;Gong, Jiansen;Cattley, Russell;Wang, Chengming;Qi, Kezong

作者机构：

关键词： colistin;Musca domestica;Protophormia terraenovae;mcr-1;mcr-2;mcr-3;resistance genes

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN： 0099-2240

年卷期： 2018 年 84 卷 1 期

页码：

收录情况： SCI

摘要： Flies have the capacity to transfer pathogens between different environments, acting as one of the most important vectors of human diseases worldwide. In this study, we trapped flies on a university campus and tested them for mobile resistance genes against colistin, a last-resort antibiotic in human medicine for treating clinical infections caused by multidrug-resistant Gram-negative bacteria. Quantitative PCR assays we developed showed that 34.1% of Musca domestica (86/252) and 51.1% of Protophormia terraenovae (23/45) isolates were positive for the mcr-1 gene, 1.2% of M. domestica (3/252) and 2.2% of P. terraenovae (2.2%, 1/45) isolates were positive for mcr-2, and 5.2% of M. domestica (13/252) and 44.4% of P. terraenovae (20/45) isolates were positive for mcr-3. Overall, 4.8% (9/189) of bacteria isolated from the flies were positive for the mcr-1 gene (Escherichia coli: 8.3%, 4/48; Enterobacter cloacae: 12.5%, 1/8; Providencia alcalifaciens: 11.8%, 2/17; Providencia stuartii: 4.9%, 2/41), while none were positive for mcr-2 and mcr-3. Four mcr-1positive isolates (two P. stuartii and two P. alcalifaciens) from blow flies trapped near a dumpster had a MIC for colistin above 4 mg/ml. This study reports mcr-1 carriage in Providencia spp. and detection of mcr-2 and mcr-3 after their initial identification in Belgium and China, respectively. This study suggests that flies might contribute significantly to the dissemination of bacteria, carrying these genes into a large variety of ecological niches. Further studies are warranted to explore the roles that flies might play in the spread of colistin resistance genes. IMPORTANCE Antimicrobial resistance is recognized as one of the most serious global threats to human health. An option for treatment of the Gram-negative ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) bacteria with multiple drug resistance was the reintroduction of the older antibiotic colistin. However, a mobile colistin resistance gene (mcr-1) has recently been found to occur widely; very recently, two other colistin resistance genes (mcr-2 and mcr-3) have been identified in Belgium and China, respectively. In this study, we report the presence of colistin resistance genes in flies. This study also reports the carriage of colistin resistance genes in the genus Providencia and detection of mcr-2 and mcr-3 after their initial identification. This study will stimulate more in-depth studies to fully elucidate the transmission mechanisms of the colistin resistance genes and their interaction.

分类号：

  • 相关文献

[1]Identification and characterization of mcr mediated colistin resistance in extraintestinal Escherichia coli from poultry and livestock in China. Yassin, Afrah Kamal,Zhang, Jilei,Wang, Jiawei,Chen, Li,Lu, Guangwu,Li, Min,Wang, Yaoyao,Wang, Chengming,Yassin, Afrah Kamal,Kelly, Patrick,Butaye, Patrick,Butaye, Patrick,Gong, Jiansen,Wei, Lanjing,Qi, Kezong,Han, Xiangan,Price, Stuart,Hathcock, Terri,Wang, Chengming. 2017

[2]Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro. Wu, Renjie,Yi, Ling-xian,Yu, Lin-feng,Wang, Jing,Liu, Yiyun,Chen, Xiaojie,Lv, Luchao,Yang, Jun,Liu, Jian-Hua,Chen, Xiaojie. 2018

[3]Molecular epidemiology of colistin-resistant Enterobacteriaceae in inpatient and avian isolates from China: high prevalence of mcr-negative Klebsiella pneumoniae. Wang, Xiaojuan,Wang, Ruobing,Jin, Longyang,Zhang, Yawei,Wang, Qi,Chen, Hongbin,Wang, Hui,Liu, Yuqing,Zhao, Min,Qi, Xiaomei,Qi, Xiaomei,Qi, Xiaomei. 2017

[4]Novel acetylcholinesterase inhibitors: Synthesis and structure-activity relationships of phthalimide alkyloxyphenyl N,N-dimethylcarbamate derivatives. Yang, Guoquan,Mei, Xiangdong,Yuan, Huizhu,Ning, Jun,Zhao, Qianfei.

[5]Quantitative and qualitative changes of the carboxylesterase associated with beta-cypermethrin resistance in the housefly, Musca domestica (Diptera: Muscidae). Shi, Jing,Shi, Xueyan,Liang, Pei,Gao, Xiwu,Gao, Junping,Zhang, Lan.

[6]Characterization of imidacloprid resistance in the housefly Musca domestica (Diptera: Muscidae). Wang, Qingmin,Gao, Xiwu,Zhang, Lan. 2012

[7]Toxicities of monoterpenes against housefly, Musca domestica L. (Diptera: Muscidae). Zhang, Zhilin,Wang, Yong,Wang, Lihua,Li, Guoyuan,Xie, Yongjian,Lin, Zhufeng.

[8]Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows. He, Tao,Wei, Ruicheng,Zhang, Lili,Sun, Lichang,Pang, Maoda,Wang, Ran,Wang, Yang.

[9]Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System. Sun, Lichang,He, Tao,Zhang, Lili,Pang, Maoda,Zhou, Yan,Bao, Hongduo,Wang, Ran,Zhang, Qiaoyan.

[10]Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319. Mu, Chun Hua,Zhang, Fa Jun,Li, Wen Cai,Lu, Shou Ping,Meng, Zhao Dong,Liu, Xia,Mu, Chun Hua,Liu, Xia,Yang, Yu,Li, Guang Cun. 2016

[11]PRSRW: AN EXPERT SYSTEM FOR POSTULATING AND INFERRING RESISTANCE GENES TO WHEAT STRIPE RUST. Yang, Yu,Wang, Lianzhi,Bian, Qiang,Xu, Shichang,Wang, Fengle. 2009

[12]Fine mapping and identification of the soybean R-SC4 resistance candidate gene to soybean mosaic virus. Wang, Dagang,Ma, Ying,Liu, Ning,Yang, Zhonglu,Zheng, Guijie,Zhi, Haijian,Wang, Dagang. 2011

[13]Genetic Analysis of Chinese Differential Cultivar Early Premium for Yellow Rust Resistance Genes. Feng, Jing,Gan, Ai-Hua,Lin, Rui-Ming,Xu, Shichang. 2011

[14]Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies. Bhatnagar, Deepak,Yu, Jiujiang,Guo, Baozhu,Fedorova, Natalie D.,Wan, Chun-Hua,Wang, Wei,Nierman, William C.,Chen, Xiaoping,Chen, Xiaoping,Nierman, William C.. 2011

[15]Multi-Drug Resistance Mediated by Class 1 Integrons in Aeromonas Isolated from Farmed Freshwater Animals. Deng, Yuting,Wu, Yali,Jiang, Lan,Tan, Aiping,Zhang, Ruiquan,Luo, Li,Wu, Yali. 2016

[16]Powdery mildew resistance in a collection of Chinese barley varieties. Dreiseitl, Antonin,Yang, Jianming. 2007

[17]Genetic mapping of bph20(t) and bph21(t) loci conferring brown planthopper resistance to Nilaparvata lugens StAyenl in rice (Oryza sativa L.). Li, R. B.,Chen, Y. Z.,Yang, L.,Li, R. B.,Chen, Y. Z.,Yang, L.,Li, R. B.,Li, Y. R.,Chen, Y. Z.,Liu, Ch.,Ma, Z. F.,Huang, D. H.,Yang, L.,Huang, F. K.,Huang, S. Sh.,Huang, L. F.,Jiang, J. J.. 2012

[18]Loci and candidate gene identification for resistance to Sclerotinia sclerotiorum in soybean (Glycine max L. Merr.) via association and linkage maps. Han, Yingpeng,Sun, Mingming,Zhao, Yue,Lv, Chunmei,Li, Dongmei,Teng, Weili,Li, Wenbin,Li, Yinghui,Qiu, Lijuan,Liu, Dongyuan,Yang, Zhijiang,Huang, Long,Zheng, Hongkun,Sun, Mingming,Lv, Chunmei.

[19]Different methods of incorporating ciprofloxacin in soil affect microbiome and degradation of ciprofloxacin residue. Liu, Longyong,Mi, Jiandui,Wang, Yan,Liao, Xindi,Wu, Yinbao,Liu, Longyong,Mi, Jiandui,Wang, Yan,Liao, Xindi,Wu, Yinbao,Liu, Longyong,Mi, Jiandui,Wang, Yan,Liao, Xindi,Wu, Yinbao,Zou, Yongde,Ma, Baohua,Liang, Juan Boo,Liu, Longyong,Mi, Jiandui,Wang, Yan,Liao, Xindi,Wu, Yinbao. 2018

[20]Large-scale identification of wheat genes resistant to cereal cyst nematode Heterodera avenae using comparative transcriptomic analysis. Wu, Du-Qing,Huang, Wen-Kun,Peng, Huan,Wang, Gao-Feng,Cui, Jiang-Kuan,Liu, Shi-Ming,Peng, De-Liang,Li, Zhi-Gang,Yang, Jun,Li, Zhi-Gang,Yang, Jun. 2015

作者其他论文 更多>>

微信小程序