Identification of the interaction and interaction domains of chicken anemia virus VP2 and VP3 proteins

文献类型: 外文期刊

第一作者: Sun, Fenfen

作者: Sun, Fenfen;Pan, Wei;Gao, Honglei;Qi, Xiaole;Qin, Liting;Wang, Yongqiang;Gao, Yulong;Wang, Xiaomei;Sun, Fenfen;Pan, Wei

作者机构:

关键词: Chicken anemia virus;VP2;VP3;Interaction;Interaction domains

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

ISSN: 0042-6822

年卷期: 2018 年 513 卷

页码:

收录情况: SCI

摘要: Chicken anemia virus (CAV) is a small, single-stranded DNA virus of Anelloviridae family. Its genome segments encode three proteins, VP1, VP2, and VP3. This study identified an interaction between VP2 and VP3 and mapped the interaction domains. Through the yeast two-hybrid (Y2H) system, VP2 was found to interact with VP3. The presence of the VP2 VP3 complex in CAV-infected chicken cells was confirmed by co-immunoprecipitation. Confocal microscopy showed that VP2 and VP3 were expressed in the cytoplasm in cotransfected Vero cells. In the Y2H system, the interaction domains were identified as being within the N-terminal aa 1-30 and C-terminal aa 17-60 for VP2 and the N-terminal aa 46-60 and C-terminal aa 1-7 for VP3. This study showed the interaction between VP2 and VP3 of CAV and identified multiple independent interactive domains within the two proteins. This provides novel information for investigating the biological functions of these proteins.

分类号:

  • 相关文献

[1]Mapping of epitopes of VP2 protein of chicken anemia virus using monoclonal antibodies. Wang, Xiaoyan,Gao, Honglei,Gao, Yulong,Fu, Chaoyang,Wang, Zhi,Lu, Guili,Cheng, Yu,Wang, Xiaomei. 2007

[2]Ribosomal protein L4 interacts with viral protein VP3 and regulates the replication of infectious bursal disease virus. Chen, Yuming,Lu, Zhen,Zhang, Lizhou,Gao, Li,Wang, Nian,Gao, Xiang,Wang, Yongqiang,Li, Kai,Gao, Yulong,Cui, Hongyu,Gao, Honglei,Liu, Changjun,Zhang, Yanping,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[3]C-terminal region of apoptin affects chicken anemia virus replication and virulence. Wang, Yongqiang,Hu, Yonghao,Chen, Hualan,Wang, Yongqiang,Song, Xiuqing,Gao, Honglei,Wang, Xiaoyan,Gao, Yulong,Qi, Xiaole,Qin, Liting,Lin, Huan,Gao, Li,Yao, Shuai,Han, Chunyan,Wang, Xiaomei,Chen, Hualan. 2017

[4]gamma gamma The chicken TH1 response: Potential therapeutic applications of ChIFTrrr. Guo, Pengju,Thomas, Jesse D.,Bruce, Matthew P.,Hinton, Tracey M.,Bean, Andrew G. D.,Lowenthal, John W.,Guo, Pengju,Thomas, Jesse D.,Bruce, Matthew P.,Hinton, Tracey M.,Guo, Pengju,Lowenthal, John W.. 2013

[5]Assessing the efficacy of an inactivated chicken anemia virus vaccine. Zhang, Xinheng,Wu, Boliang,Chen, Weiguo,Dai, Zhenkai,Bi, Yingzuo,Xie, Qingmei,Zhang, Xinheng,Wu, Boliang,Liu, Yuanjia,Bi, Yingzuo,Xie, Qingmei,Liu, Yuanjia,Chen, Weiguo,Xie, Qingmei,Chen, Weiguo,Dai, Zhenkai,Xie, Qingmei.

[6]Antigenic structure analysis of VP3 of infectious bursal disease virus. Deng, Xiaoyun,Gao, Yulong,Gao, Honglei,Qi, Xiaole,Cheng, Yu,Wang, Xiaoyan,Wang, Xiaomei. 2007

[7]Kobuvirus VP3 protein restricts the IFN-beta-triggered signaling pathway by inhibiting STAT2-IRF9 and STAT2-STAT2 complex formation. Peng, Qianqian,Lan, Xi,Wang, Chen,Wang, Junyong,Zhu, Qiyun,Ren, Yujie,Yue, Ningning,Zhong, Bo,Zhong, Bo. 2017

[8]A single amino acid in the C-terminus of VP3 protein influences the replication of attenuated infectious bursal disease virus in vitro and in vivo. Wang, Yongqiang,Qi, Xiaole,Kang, Zhonghui,Yu, Fei,Qin, Liting,Gao, Honglei,Gao, Yulong,Wang, Xiaomei. 2010

[9]Generation and evaluation of a recombinant genotype VII Newcastle disease virus expressing VP3 protein of Goose parvovirus as a bivalent vaccine in goslings. Wang, Jianzhong,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Wang, Jianzhong,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Wang, Chunfeng,Feng, Na,Yang, Songtao,Xia, Xianzhu,Xiao, Yueqiang,Wang, Wenxiu,Liu, Xiufan,Hu, Shunlin,Ding, Chan,Yu, Shengqing.

[10]Function of VP2 Protein in the Stability of the Secondary Structure of Virus-like Particles of Genogroup II Norovirus at Different pH Levels: Function of VP2 Protein in the Stability of NoV VLPs. Yao Lin,Li Fengling,Wang Lianzhu,Zhai Yuxiu,Jiang Yanhua. 2014

[11]The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection. Zhang, Lizhou,Li, Hui,Chen, Yuming,Gao, Xiang,Lu, Zhen,Gao, Li,Wang, Yongqiang,Gao, Yulong,Gao, Honglei,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Pan, Qing,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[12]Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens. Liu, Linlin,Zhang, Wang,Song, Yuxin,Wang, Wenqian,Zhang, Yuan,Wang, Tingting,Li, Kai,Pan, Qing,Qi, Xiaole,Gao, Yulong,Gao, Li,Liu, Changjun,Zhang, Yanping,Wang, Yongqiang,Wang, Xiaomei,Cui, Hongyu,Zhang, Wang,Song, Yuxin,Zhang, Yuan,He, Gaoming. 2018

[13]A single mutation in the P-BC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus. Qi, Xiaole,Gao, Xiang,Lu, Zhen,Zhang, Lizhou,Wang, Yongqiang,Gao, Li,Gao, Yulong,Li, Kai,Gao, Honglei,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Wang, Xiaomei,Wang, Xiaomei. 2016

[14]Identification of a serotype-independent linear epitope of foot-and-mouth disease virus. Yang, Baolin,Wang, Mingxia,Liu, Wenming,Xu, Zhiqiang,Wang, Haiwei,Yang, Decheng,Zhou, Guohui,Yu, Li,Ma, Wenge. 2017

[15]Effects of amino acid substitutions in the VP2 B-C loop on antigenicity and pathogenicity of serotype Asia1 foot-and-mouth disease virus. Xue, Mei,Wang, Haiwei,Li, Wan,Zhou, Guohui,Tu, Yabin,Yu, Li. 2012

[16]Typing of Canine Parvovirus Strains Circulating in North-East China. Zhao, H.,Wang, J.,Cheng, Y.,Lin, P.,Zhu, H.,Yi, L.,Zhang, S.,Guo, L.,Cheng, S.,Jiang, Y.,Han, G..

[17]Naturally occurring mutations at residues 253 and 284 in VP2 contribute to the cell tropism and virulence of very virulent infectious bursal disease virus. Qi, Xiaole,Gao, Honglei,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Gao, Li,Wang, Xiaomei,Qi, Xiaole. 2009

[18]Molecular epidemiology of Aleutian mink disease virus in China. Wang, Zhenjun,Wu, Wei,Hu, Bo,Zhang, Hailing,Bai, Xue,Zhao, Jianjun,Zhang, Lei,Yan, Xijun.

[19]Fine Mapping of a Foot-and-mouth Disease Virus Epitope Recognized by Serotype-Independent Monoclonal Antibody 4B2. Yu, Yongzhong,Wang, Haiwei,Zhao, Lei,Zhang, Chunyuan,Jiang, Zhigang,Yu, Li.

[20]Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus. Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Tan, Yeping.

作者其他论文 更多>>