Lactoferrin Exerts Antitumor Effects by Inhibiting Angiogenesis in a HT29 Human Colon Tumor Model

文献类型： 外文期刊

第一作者： Li, Hui-Ying

作者： Li, Hui-Ying;Li, Ming;Luo, Chao-chao;Wang, Jia-Qi;Zheng, Nan

作者机构：

关键词： lactoferrin;HT29 cell;HCT8 cell;tumor-bearing model;angiogenesis

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2017 年 65 卷 48 期

页码：

收录情况： SCI

摘要： To investigate the effect and potential mechanisms of lactoferrin on colon cancer cells and tumors, HT29 and HCT8 cells were exposed to varying concentrations of lactoferrin, and the impacts on cell proliferation, migration, and invasion were observed. Cell proliferation test showed that high dosage of lactoferrin (5-100 mg/mL) inhibited cell viability in a dose dependent manner, with the 50% concentration of inhibition at 81.3 +/- 16.7 mg/mL and 101 +/- 23.8 mg/mL for HT29 and HCT8 cells, respectively. Interestingly, migration and invasion of the cells were inhibited dramatically by 20 mg/mL lactoferrin, consistent with the significant down regulation of VEGFR2, VEGFA, pPI3K, pAkt, and pErk1/2 proteins. HT29 was chosen as the sensitive cell line to construct a tumor-bearing nude mice model. Notably, HT29 tumor weight was greatly reduced in both the lactoferrin group (26.5 +/- 6.7 mg) and the lactoferrin/S-Fu group (14.5 +/- 5.1 mg), compared with the control one (39.3 +/- 6.5 mg), indicating that lactoferrin functioned as a tumor growth inhibitor. Considering lactoferrin also reduced the growth of blood vessels and the degree of malignancy, we concluded that HT29 tumors were effectively suppressed by lactoferrin, which might be achieved by regulation of phosphorylation from various kinases and activation of the VEGFR2-PI3K/Akt-Erk1/2 pathway.

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