MicroRNA-34c acts as a bidirectional switch in the maturation of insulin-producing cells derived from mesenchymal stem cells

文献类型： 外文期刊

第一作者： Bai, Chunyu

作者： Bai, Chunyu;Yang, Wancai;Gao, Yuhua;Zhang, Xiangyang;Bai, Chunyu;Gao, Yuhua;Guan, Weijun;Yang, Wancai

作者机构：

关键词： insulin-producing cell;mesenchymal stem cells;differentiation;miR-34c;insulin secretion

期刊名称：ONCOTARGET （ 影响因子：5.168； 五年影响因子：5.312 ）

ISSN： 1949-2553

年卷期： 2017 年 8 卷 63 期

页码：

收录情况： SCI

摘要： miRNAs regulate insulin secretion, pancreatic development, and beta-cell differentiation. However, their function in the differentiation of IPCs from MSCs is poorly understood. In this study, to screen for miRNAs and their targets that function during the formation of IPCs from MSCs, we examined the miRNA expression profiles of MSCs and IPCs using RNA-seq and qPCR to confirm the above results. We found that miR-34c exhibited transient upregulation at an early stage of the formation of IPCs derived from MSCs. Next, we analyzed the biological function of miR-34c by predicting its targets using bioinformatic tools. Combining our data with those from previous reports, we found that miR-34c and its targets play an important role in the formation of IPCs. Therefore, we overexpressed miR-34c and expressed small interfering RNAs of its targets in MSCs to investigate their functions in IPC formation. We found that miR-34c acts as a bidirectional switch in the formation of IPCs derived from MSCs by regulating the expression of targets to affect insulin synthesis and secretion. miR-34c was shown to downregulate its targets, including PDE7B, PDGFRA, and MAP2K1, to increase proinsulin synthesis, but when miR-34c continually dysregulated such expression, it suppressed the expression of other targets, namely ACSL4 and SAR1A, weakening insulin secretion in IPCs. These results suggest that endogenous miRNAs involved in the formation of IPCs from stem cells should be considered in the development of effective cell transplant therapy for diabetes.

分类号：

  • 相关文献

[1]MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells. Bai, Chunyu,Gao, Yuhua,Li, Xiangchen,Wang, Kunfu,Xiong, Hui,Shan, Zhiqiang,Zhang, Ping,Wang, Wenjie,Guan, Weijun,Ma, Yuehui. 2017

[2]Generation of insulin-secreting cells from adult rat pancreatic ductal epithelial cells induced by hepatocyte growth factor and betacellulin-delta 4. Zhan, Xiao-Rong,Li, Xin-Yu,Liu, Xiao-Min,Yang, Yu-Ling,Yi, Ran,Zhang, Jing,Yang, Bao-Feng,Zhou, Jian-Hua. 2009

[3]Biological characterization of mesenchymal stem cells from bovine umbilical cord. Xiong, Hui,Bai, Chunyu,Wu, Shuang,Gao, Yuhua,Lu, Taofeng,Guan, Weijun,Ma, Yuehui,Xiong, Hui,Hu, Qingyun.

[4]Differentiation potential of bone marrow mesenchymal stem cells in duck. Li, Linfeng,Gong, Xuelian,Liu, Hongkun,Chen, Lina,Guan, Weijun,Ma, Yuehui,Li, Linfeng,Bai, Xiujuan. 2009

[5]Role of microRNA-21 in the formation of insulin -producing cells from pancreatic progenitor cells. Bai, Chunyu,Li, Xiangchen,Gao, Yuhua,Wang, Kunfu,Fan, Yanan,Zhang, Shuang,Ma, Yuehui,Guan, Weijun.

[6]Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. Zhang, Tianshi,Kong, Jie,Wang, Weiji,Wang, Qingyin,Zhang, Tianshi.

[7]Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos. Cher, SL,Sha, ZX,Ye, HQ.

[8]Isolation and characterization of mesenchymal stem cells from chicken bone marrow. Bai, Chunyu,Ma, Yuehui,Chen, Lina,Guan, Weijun,Bai, Chunyu,Zhang, Minghai,Hou, Lingling. 2013

[9]Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration. Li, Yang,Liu, Ming-yuan,Wang, Xue-lin,Han, Wen-yu,Liu, Ya-hui,Suo, Jian,Li, Zhi-jie,Li, Ya-gang,Jin, Hu. 2013

[10]Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous Mats. Luo, Yu,Shen, He,Cao, Yuhua,Huang, Jie,Zhang, Mengxin,Dai, Jianwu,Zhang, Zhijun,Luo, Yu,Shi, Xiangyang,Fang, Yongxiang,Dai, Jianwu,Fang, Yongxiang. 2015

[11]Wip1 knockout inhibits the proliferation and enhances the migration of bone marrow mesenchymal stem cells. Tang, Yiting,Sheng, Ming,Liu, Honglin,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Xiong, Kai. 2015

[12]Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells. Bai, Chunyu,Gao, Yuhua,Li, Qian,Feng, Yuan,Yu, Yanze,Meng, Gentong,Zhang, Minghai,Bai, Chunyu,Gao, Yuhua,Li, Qian,Guan, Weijun.

[13]Inhibitory effect and mechanism of mesenchymal stem cells on liver cancer cells. Hou, Lingling,Wang, Xiaoyu,Zhou, Yaqiong,Ma, Haibin,Wang, Ziling,He, Jinsheng,Hu, Honggang,Guan, Weijun,Ma, Yuehui. 2014

[14]MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting multifunctional protein 2. Li, Biao,Xie, Shanshan,Cai, Chunbo,Qian, Lili,Jiang, Shengwang,Ma, Dezun,Xiao, Gaojun,Gao, Ting,Cui, Wentao,Yang, Jinzeng. 2017

[15]Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Jin, C,Wang, PS,Xia, YM,Xu, M,Pei, SJ. 2005

[16]Isolation and Characterization of Mesenchymal Stem Cells Derived from Duck Embryo Liver. Wang, Wenjie,Guan, Weijun,Ma, Yuehui,Huang, Hongmei,Li, Qian,Wang, KunFu,Chen, Jia. 2017

[17]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

[18]Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species. Jin, Shumei,Wang, Ji,Sun, Dan,Liu, Shengkui,Wang, Xinwang,Genovesi, A. D.,Li, Guoliang. 2014

[19]Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro. Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui,Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui,Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui. 2016

[20]IGF1 regulates RUNX1 expression via IRS1/2: Implications for antler chondrocyte differentiation. Yang, Zhan-Qing,Zhang, Hong-Liang,Geng, Shuang,Wang, Kai,Yu, Hai-Fan,Yue, Zhan-Peng,Guo, Bin,Duan, Cui-Cui. 2017

作者其他论文 更多>>

微信小程序