The double-antigen ELISA concept for early detection of E-rns-specific classical swine fever virus antibodies and application as an accompanying test for differentiation of infected from marker vaccinated animals

文献类型： 外文期刊

第一作者： Meyer, D.

作者： Meyer, D.;Becher, P.;Postel, A.;Fritsche, S.;Engemann, C.;Luo, Y.;Qiu, H. -J.;Blome, S.;Beyerbach, M.;Chang, C. -Y.

作者机构：

关键词： classical swine fever virus;DIVA assay;DIVA vaccine;double-antigen ELISA;E-rns-based antibody ELISA;serology

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：5.005； 五年影响因子：4.622 ）

ISSN： 1865-1674

年卷期： 2017 年 64 卷 6 期

页码：

收录情况： SCI

摘要： Emergency vaccination with live marker vaccines represents a promising control strategy for future classical swine fever (CSF) outbreaks, and the first live marker vaccine is available in Europe. Successful implementation is dependent on a reliable accompanying diagnostic assay that allows differentiation of infected from vaccinated animals (DIVA). As induction of a protective immune response relies on virus-neutralizing antibodies against E2 protein of CSF virus (CSFV), the most promising DIVA strategy is based on detection of E-rns-specific antibodies in infected swine. The aim of this study was to develop and to evaluate a novel E-rns-specific prototype ELISA (pigtype CSFV E-rns Ab), which may be used for CSF diagnosis including application as an accompanying discriminatory test for CSFV marker vaccines. The concept of a double-antigen ELISA was shown to be a solid strategy to detect E-rns-specific antibodies against CSFV isolates of different genotypes (sensitivity: 93.5%; specificity: 99.7%). Furthermore, detection of early seroconversion is advantageous compared with a frequently used CSFV E2 antibody ELISA. Clear differences in reactivity between sera taken from infected animals and animals vaccinated with various marker vaccines were observed. In combination with the marker vaccine CP7_E2alf, the novel ELISA represents a sensitivity of 90.2% and a specificity of 93.8%. However, cross-reactivity with antibodies against ruminant pestiviruses was observed. Interestingly, the majority of samples tested false-positive in other E-rns-based antibody ELISAs were identified correctly by the novel prototype E-rns ELISA and vice versa. In conclusion, the pigtype CSFV E-rns Ab ELISA can contribute to an improvement in routine CSFV antibody screening, particularly for analysis of sera taken at an early time point after infection and is applicable as a DIVA assay. An additional E-rns antibody assay is recommended for identification of false-positive results in a pig herd immunized with the licensed CP7_E2alf marker vaccine.

分类号：

  • 相关文献

[1]Development and Preliminary Application of an Indirect ELISA to Detect Infectious Bovine Rhinotracheitis Virus Using Recombinant Glycoprotein D of IBRV Strain SD. Song, Lingling,Zhang, Hui,Hou, Peili,Wang, Hongmei,Zhao, Guimin,He, Hongbin,Xia, Xianzhu. 2016

[2]A monoclonal antibody-blocking enzyme-linked immunosorbent assay for the detection of serovar-specific antibodies to Haemophilus paragallinarum. Zhang, P,Blackall, PJ,Yamaguchi, T,Iritani, Y. 1999

[3]Identification and characterization of a new cucumber mosaic virus isolate from passionfruit in Hainan of China. Liu, ZX,Pan, JS,Wei, YW,Wu, H,Zheng, XQ. 2004

[4]First report of Rickettsia felis in China. Zhang, Jilei,Lu, Guangwu,Wei, Lanjing,Wang, Chengming,Kelly, Patrick,Zhang, Zhenwen,Yu, Duonan,Kayizha, Shayilan. 2014

[5]Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape. Cai, Li,Hou, Mingsheng,Cai, Li,Chen, Kunrong,Zhang, Xuejiang,Yan, Liying,Xu, Zeyong. 2009

[6]Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection. Feng, Zhi-Xin,Bai, Yun,Yao, Jing-Ting,Gan, Yuan,Wang, Hai-Yan,Wei, Yan-Na,Liu, Mao-Jun,Xiong, Qi-Yan,Bai, Fang-Fang,Li, Bin,Wu, Xu-Su,Shao, Guo-Qing,Feng, Zhi-Xin,Bai, Yun,Liu, Mao-Jun,Shao, Guo-Qing,Pharr, G. Todd,Wan, Xiu-Feng,Xiao, Shao-Bo,Chi, Ling-Zhi.

[7]Characterization and partial sequence of a new furovirus of wheat in China. Ye, R,Zheng, T,Chen, J,Diao, A,Adams, MJ,Yu, S,Antoniw, JF.

[8]Genome and Molecular Characterization of a CSFV Strain Isolated from a CSF Outbreak in South China. Shen, Hai-Yan,Wang, Jia-Ying,Dong, Xiao-Ying,Zhao, Ming-Qiu,Kang, Yanmei,Li, Yin-Guang,Pei, Jing-Jing,Liao, Ming,Ju, Chun-Mei,Yi, Lin,Hu, Yongming,Chen, Jin-Ding,Shen, Hai-Yan. 2013

[9]In vitro inhibition of the replication of classical swine fever virus by capsid-targeted virus inactivation. Wang, Yu-Fei,Wang, Zhong-Hua,Li, Yan,Zhang, Xing-Juan,Sun, Yuan,Li, Miao,Qiu, Hua-Ji. 2010

[10]Identification of host cell binding peptide from an overlapping peptide library for inhibition of classical swine fever virus infection. Li, Xuewu,Wang, Li,Zhao, Dong,Zhang, Gaiping,Luo, Jun,Deng, Ruiguang,Yang, Yanyan. 2011

[11]The protective immune response induced by B cell epitope of classical swine fever virus glycoprotein E2. Liu, SG,Tu, CC,Wang, CL,Yu, XL,Wu, JM,Guo, SP,Shao, ML,Gong, Q,Zhu, QH,Kong, XG. 2006

[12]Generation of a Recombinant Baculovirus Expressing The E2 Protein of Classical Swine Fever Virus and Its Immunogenicity in a Mouse Model. Miao, Li,Wang, Yu-Fei,Yu, Wang,Hui, Gao,Na, Li,Yuan, Sun,Liang, Bing-Bing,Qiu, Hua-Ji. 2009

[13]A multiplex nested RT-PCR for the detection and differentiation of wild-type viruses from C-strain vaccine of classical swine fever virus. Li, Yan,Zhao, Jian-Jun,Li, Na,Shi, Zixue,Cheng, Dan,Zhu, Qing-Hu,Tu, Changchun,Tong, Guang-Zhi,Qiu, Hua-Ji. 2007

[14]RNA interference screening of interferon-stimulated genes with antiviral activities against classical swine fever virus using a reporter virus. Wang, Xiao,Li, Yongfeng,Li, Lian-Feng,Shen, Liang,Zhang, Lingkai,Yu, Jiahui,Luo, Yuzi,Sun, Yuan,Li, Su,Qiu, Hua-Ji.

[15]Specific ligands for classical swine fever virus screened from landscape phage display library. Yin, Long,Luo, Yuzi,Du, Min,Qiu, Hua-Ji,Yin, Long,Liang, Bo,Wang, Fei,Liu, Aihua,Yin, Long,Wang, Fei,Liu, Aihua,Petrenko, Valery A..

[16]Development and evaluation of rapid detection of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Yin, Shuanghui,Shang, Youjun,Zhou, Guangqing,Tian, Hong,Liu, Yanhong,Cai, Xuepeng,Liu, Xiangtao.

[17]Development of a triplex TaqMan real-time RT-PCR assay for differential detection of wild-type and HCLV vaccine strains of classical swine fever virus and bovine viral diarrhea virus 1. Zhang, Xing-Juan,Han, Qiu-Ying,Sun, Yuan,Zhang, Xin,Qiu, Hua-Ji.

[18]Pre-Clinical Evaluation of a Real-Time PCR Assay on a Portable Instrument as a Possible Field Diagnostic Tool: Experiences from the Testing of Clinical Samples for African and Classical Swine Fever Viruses. Liu, L.,Stahl, K.,Belak, S.,Liu, L.,Stahl, K.,Belak, S.,Liu, L.,Luo, Y.,Qiu, H. -J.,Luo, Y.,Qiu, H. -J.,Accensi, F.,Ganges, L.,Rodriguez, F.,Accensi, F.,Rodriguez, F.,Shan, H.,Belak, S..

[19]Efficacy evaluation of the C-strain-based vaccines against the subgenotype 2.1d classical swine fever virus emerging in China. Luo, Yuzi,Ji, Shengwei,Lei, Jian-Lin,Xian, Guang-Tao,Liu, Yan,Gao, Yao,Meng, Xing-Yu,Zheng, Guanglai,Zhang, En-Yu,Wang, Yimin,Du, Ming-Liang,Li, Yongfeng,Li, Su,He, Xi-Jun,Sun, Yuan,Qiu, Hua-Ji.

[20]Enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored DNA vaccine and a recombinant adenovirus. Sun, Yuan,Li, Na,Li, Hong-Yu,Li, Miao,Qiu, Hua-Ji. 2010

作者其他论文 更多>>

微信小程序