Development of a blocking ELISA for Caprine parainfluenza virus type

文献类型: 外文期刊

第一作者: Mao, Li

作者: Mao, Li;Li, Wenliang;Zhou, Tianci;Yang, Leilei;Hao, Fei;Li, Jizong;Zhang, Wenwen;Jiang, Jieyuan;Mao, Li;Luo, Xuenong

作者机构:

关键词: Caprine parainfluenza virus type 3;Blocking ELISA;Monoclonal antibodies;Hybridoma screening

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN: 0166-0934

年卷期: 2017 年 250 卷

页码:

收录情况: SCI

摘要: Caprine parainfluenza virus type 3 (CPIV3) is a novel pathogen mainly causing respiratory diseases in goats. At present, there are no high throughput and rapid testing methods available for epidemiological investigation. In this study, we designed a modified method for selection of hybridomas that secrete monoclonal antibodies (mAb) specific for CPIV3. The monoclonal antibodies were obtained by combination of indirect enzyme-linked immunosorbent assay (iELISA) and blocking ELISA (bELISA). The technique was efficient to determine each mAb with specificity and sensitivity. One bELISA was validated for the serological diagnosis of CPIV3. After optimization conditions were established, a total of 205 reference goat sera were tested in parallel by bELISA and by virus neutralization (VN) for their relative performances. The cut-off point was ultimately defined as 33.6% by ROC curve analysis. The bELISA specificity and sensitivity were 99.2% and 98.7%, respectively, and agreement with the VN test was > 99.0%. Furthermore, testing another 2919 goat sera by bELISA demonstrated 39.3% prevalence in the goat population, more sensitive than HI detection. This new bELISA would offer higher throughput, sensitivity, and specific detection for CPIV3, and will be of great value not only for surveillance, but also for monitoring the efficiency of vaccination programs in the future.

分类号:

  • 相关文献

[1]Pathogenicity and horizontal transmission studies of caprine parainfluenza virus type 3 JS2013 strain in goats. Li, Wenliang,Hao, Fei,Mao, Li,Wang, Zhongyu,Zhou, Tianci,Deng, Jiawu,Li, Jizong,Zhang, Wenwen,Yang, Leilei,Jiang, Jieyuan,Wang, Zhongyu,Lv, Yingjun,Zhou, Tianci,Deng, Jiawu.

[2]Rapid detection of novel caprine parainfluenza virus type 3 (CPIV3) using a TaqMan-based RT-qPCR. Li, Jizong,Li, Wenliang,Mao, Li,Hao, Fei,Yang, Leilei,Zhang, Wenwen,Jiang, Jieyuan.

[3]Development of a blocking ELISA for detection of antibodies against avian hepatitis E virus. Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhang, Gaiping,Zhou, En-Min,Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhou, En-Min,Zhang, Gaiping,Luo, Jianxuri,Hsu, Walter H..

[4]A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens. Sun, H.,Miao, D.,Zhang, P.,Gong, Y.,Blackall, P. J.. 2007

[5]Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. Liu, Maojun,Zhang, Yue,Wu, Yuzi,Wang, Haiyan,Li, Bin,Bai, Yun,Feng, Zhixin,Xiong, Qiyan,Bai, Fangfang,Shao, Guoqing,Liu, Maojun,Du, Gaimei,Browning, Glenn F.,Shao, Guoqing.

[6]Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. Huang, Liping,Lu, Yuehua,Wei, Yanwu,Guo, Longjun,Liu, Changming. 2011

[7]Development of blocking ELISA for detection of antibodies against H9N2 avian influenza viruses. Li, Zejun.

[8]Immunohistochemical analysis of cell wall hydroxyproline-rich glycoproteins in the roots of resistant and susceptible wax gourd cultivars in response to Fusarium oxysporum f. sp Benincasae infection and fusaric acid treatment. Ma, Li,Xu, Chunxiang,Xie, Dasen,Samaj, Jozef. 2011

[9]Production and evaluation of a panel of monoclonal antibodies against Haemophilus paragallinarum. Zhang, P,Blackall, PJ,Yamaguchi, T,Iritani, Y. 2000

[10]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[11]Generation and characterization of monoclonal antibodies specific for 18 kDa antigen from Taenia solium cysticerci. Zhang, Shaohua,Luo, Xuenong,Guo, Aijiang,Zhu, Xueliang,Cai, Xuepeng.

[12]Development of a Rapid Immunochromatographic Lateral Flow Device Capable of Differentiating Phytase Expressed from Recombinant Aspergillus niger phyA2 and Genetically Modified Corn. Zhou, Xiaojin,Pu, Ling-Kui,Hui, Elizabeth,Zhang, Jun,Yu, Xiao-Lin,Tu, Zhiguan,Lin, Zhen,Zheng, Jian,Zhang, Jun,Liu, Oi,Liu, Oi,Zhane, Juan.

[13]Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Du, Wenjuan,Wang, Yiping,Huang, Liping,Wei, Yanwu,Chen, Dongjie,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[14]New Monoclonal Antibodies against a Novel Subtype of Shiga Toxin 1 Produced by Enterobacter cloacae and Their Use in Analysis of Human Serum. Skinner, Craig,Patfield, Stephanie,Khalil, Rowaida,Kong, Qiulian,He, Xiaohua,Khalil, Rowaida,Kong, Qiulian. 2016

[15]Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection. Zhao, Liang,Li, Jiaying,Li, Ye,Wang, Tiangang,Rahman, Ebeydulla,Ji, Baoping,Zhou, Feng,Jin, Xiaolu,Wang, Kai,Xing, Yuan,Xing, Yuan.

[16]Antigenic subtyping and epitopes' competition analysis of porcine circovirus type 2 using monoclonal antibodies. Saha, Dipongkor,Huang, Liping,Bussalleu, Eva,Lefebvre, David J.,Fort, Maria,Nauwynck, Hans J.,Huang, Liping,Van Doorsselaere, Jan.

[17]Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure. Li, Peiwu,Li, Peiwu,Zhang, Qi,Zhang, Wen,Huang, Yanling,Ding, Xiaoxia,Jiang, Jun.

[18]Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Ren, Jian-Le,Zhu, Yuan-Mao,Zhou, Yue-Hui,Lv, Chuang,Yan, Hao,Ma, Lei,Shi, Hong-Fei,Xue, Fei.

[19]Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease. Lin, Tong,Shao, Jun-jun,Du, Jun-zheng,Cong, Guo-zheng,Gao, Shan-dian,Chang, Huiyun. 2011

[20]Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16. Wang, Wen-Shi,Sun, En-cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai. 2013

作者其他论文 更多>>