ElNFS1, a nitroreductase gene from Enterobacter ludwigii, confers enhanced detoxification and phytoremediation of 4-nitrobenzaldehyde in rice

文献类型： 外文期刊

第一作者： Li, Zhenjun

作者： Li, Zhenjun;Gao, Jianjie;Tian, Yongsheng;Wang, Bo;Xu, Jing;Fu, Xiaoyan;Han, Hongjuan;Wang, Lijuan;Zhang, Wenhui;Wang, Yu;Deng, Yongdong;Gong, Zehao;Peng, Rihe;Yao, Quanhong

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关键词： 4-Nitrobenzaldehyde; Nitroreductase; Photosynthetic apparatus; Phytoremediation; ROS

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：9.988； 五年影响因子：10.366 ）

ISSN： 0269-7491

年卷期： 2022 年 314 卷

页码：

收录情况： SCI

摘要： 4-nitrobenzaldehyde (4-NBA) is a widely used chemical intermediate for industrial application and an important photodegradation product of chloramphenicol. This compound represents a substantial threat to human health and ecosystem due to its genotoxic and mutagenic effect. In this study, the 4-NBA detoxification by transgenic rice overexpressing a bacterial nitroreductase gene, ElNFS1, from Enterobacter ludwigii were investigated. The cytosol-targeted ElNFS1 transgenic plants were selected to comprehensively examine their physio-biochemical responses and phytoremediation potential to 4-NBA. Our results showed that the transgenic plants exhibited strong tolerance to 4-NBA. Overexpression of ElNFS1 could significantly alleviate 4-NBA-induced damages of photosynthetic apparatus and reactive oxygen species overproduction in transgenic plants. The phytor-emediation assay revealed that transgenic plants could remove more 4-NBA from the medium than wild-type plants. HPLC and LC-MS assays showed that 4-aminobenzaldehyde was found in the reductive products of 4 -NBA. Altogether, the function of ElNFS1 during 4-NBA detoxification was characterized for the first time, which provides a strong theoretical support for the application potential of ElNFS1 transgenic plants on the phytoremediation of 4-NBA.

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