Transcriptome and Metabolome Analyses Provide Insights into the Occurrence of Peel Roughing Disorder on Satsuma Mandarin (Citrus unshiu Marc.) Fruit

文献类型： 外文期刊

第一作者： Li, Fei-Fei

作者： Li, Fei-Fei;Xiong, Jiang;Cao, Xiong-Jun;Ma, Xiao-Chuan;Zhang, Zi-Mu;Xie, Shen-Xi;Lu, Xiao-Peng;Li, Fei-Fei;Xiong, Jiang;Cao, Xiong-Jun;Ma, Xiao-Chuan;Zhang, Zi-Mu;Xie, Shen-Xi;Li, Fei-Fei;Cao, Shang-Yin

作者机构：

关键词： satsuma mandarin;roughing disorder;GA;starch;mineral nutrients;RNA-sequencing;metabolome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Roughing disorder (RD) is a significant quality barrier in citrus fruit, prevalent on easy-peeling mandarins. As RD is not yet well-understood, this study aimed to examine the changes and synergic molecular processes involved in peel RD. Peel with RD was induced by severely defruiting Satsuma mandarin trees. Morphology observations, RNA-sequencing, and targeted and untargeted metabolic analyses were conducted. The results showed that the primary metabolites of sugars, organic acids and amino acids are dramatically changed in RD peel. The RD peel was always characterized by higher magnesium content during development. Comparative transcriptome profiling was performed for CK and RD peels at 30, 80, and 170 days after full bloom(DAFB) which represented fruit at cell division stage, cell enlargement stage and fruit maturity stage, respectively. Physiological and molecular biological evidence suggested that the month after full bloom is a crucial stage for RD initiation. A total of 4,855 differentially expressed genes (DEGs) in RD peel, relative to CK peel were detected at cell division stage, about 2 to 4-fold more than other stages had. Among the differentially expressed transcription factors, the bHLH family were affected most by RD, and six bHLH transcription factors functionally involved in GA metabolism were assessed to associate with RD occurrence. Gene set enrichment analysis suggested that RD significantly altered starch and GA metabolism in peel. Higher starch content and hydrolysed chain status were found in RD peel at cell division stage. RD occurrence on the peel was influenced significantly by GA, especially abundant GA before July. These changes may mean a significant alteration in sink strength of RD peel. The findings of this study provide insights into the emergence, development and molecular mechanisms of RD.

分类号：

  • 相关文献

[1]Antioxidative systems, metal ion homeostasis and cadmium distribution in Iris lactea exposed to cadmium stress. Guo, Qiang,Meng, Lin,Zhang, Ya-Nan,Mao, Pei-Chun,Tian, Xiao-Xia,Li, Shan-Shan,Zhang, Lin. 2017

[2]Role of exogenous salicylic acid in alleviating cadmium-induced toxicity in Kentucky bluegrass. Guo, Qiang,Meng, Lin,Mao, Pei-Chun,Jia, Yan-Qiong,Shi, Yong-Jie.

[3]Arbuscular mycorrhizal fungi associated with citrus orchards under different types of soil management, southern China. Wang, P.,Zhang, J. J.,Shu, B.,Xia, R. X.,Wang, P.. 2012

[4]Fruit Photosynthesis and Assimilate Translocation and Partitioning: Their Characteristics and Role in Sugar Accumulation in Developing Citrus unshiu Fruit. Chen, JW,Zhang, SL,Zhang, LC,Zhao, ZZ,Xu, JG.

[5]Studying the Differences of Bacterial Metabolome and Microbiome in the Colon between Landrace and Meihua Piglets. Yan, Shijuan,Zhu, Cui,Yu, Ting,Huang, Wenjie,Huang, Jianfeng,Kong, Qian,Shi, Jingfang,Chen, Zhongjian,Liu, Qinjian,Wang, Shaolei,Jiang, Zongyong,Chen, Zhuang,Huang, Jianfeng,Jiang, Zongyong. 2017

[6]Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses. Tang, Chaohua,Zhang, Kai,Zhan, Tengfei,Zhao, Qingyu,Zhang, Junmin,Tang, Chaohua,Zhang, Kai,Zhan, Tengfei,Zhao, Qingyu,Zhang, Junmin.

[7]Zinc stress affects ionome and metabolome in tea plants. Zhang, Yinfei,Wang, Yu,Ding, Zhaotang,Jia, Sisi,Ma, Dexin,Wang, Hui,Song, Lubin.

[8]Integrated omics data of two annual ryegrass (Lolium multiflorum L.) genotypes reveals core metabolic processes under drought stress. Pan, Ling,Ma, Xiao,Yang, Zhongfu,Zhang, Xinquan,Meng, Chen,Wang, Jianping,Fan, Xiaomei,Zhou, Meiliang. 2018

[9]The Development of Microbiota and Metabolome in Small Intestine of Sika Deer (Cervus nippon) from Birth to Weaning. Li, Zhipeng,Wang, Xiaoxu,Zhang, Ting,Si, Huazhe,Nan, Weixiao,Xu, Chao,Li, Guangyu,Guan, Leluo,Wright, Andre-Denis G.. 2018

[10]Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis. Chen, Xiao-Qing,Hu, Gui-Xue,Chen, Xiao-Qing,Zhou, Chun-Xue,He, Shuai,Zhu, Xing-Quan,Zhou, Chun-Xue,Elsheikha, Hany M.,He, Shuai. 2017

[11]Study on the optimal algorithm prediction of corn leaf component information based on hyperspectral imaging. Wu, Qiong,Xu, Tongyu,Wu, Qiong,Wang, Jihua,Wang, Cheng. 2016

[12]Soaking in H2O2 regulates ABA biosynthesis and GA catabolism in germinating cotton seeds under salt stress. Kong, Xiangqiang,Luo, Zhen,Zhang, Yanjun,Li, Weijiang,Dong, Hezhong.

[13]Optimizing an Electronic Nose for Analysis Honey from Different Nectar Sources. Liu, Ningjing,Qing, Zhaoshen,Ji, Baopin,Zhou, Feng,Shi, Bolin,Zhao, Lei,Tian, Wenli.

[14]Developing a multispectral imaging for simultaneous prediction of freshness indicators during chemical spoilage of grass carp fish fillet. Cheng, Jun-Hu,Sun, Da-Wen,Qu, Jia-Huan,Pu, Hong-Bin,Cheng, Jun-Hu,Sun, Da-Wen,Qu, Jia-Huan,Pu, Hong-Bin,Sun, Da-Wen,Zhang, Xiao-Chao,Song, Zhongxiang,Chen, Xinghai,Zhang, Hong.

[15]ZmGRF, a GA regulatory factor from maize, promotes flowering and plant growth in Arabidopsis. Xu, Miaoyun,Yang, Hongmei,Hu, Zhiqiu,Hu, Xiaolong,Luan, Mingda,Zhang, Lan,Fan, Yunliu,Wang, Lei,Lu, Yunming,He, Jingcheng,Yang, Hongmei,Hu, Zhiqiu,Hu, Xiaolong,Luan, Mingda.

[16]Nitric oxide improves aluminum tolerance by regulating hormonal equilibrium in the root apices of rye and wheat. He, Long-Fei,Gu, Ming-Hua,Li, Xiao-Feng,He, Hu-Yi. 2012

[17]Identification of a novel tillering dwarf mutant and fine mapping of the TDDL(T) gene in rice (Oryza sativa L.). Gao ZhenYu,Guo LongBiao,Liu Jian,Dong GuoJun,Hu Jiang,Qian Qian,Gao ZhenYu,Liu XiaoHui,Han Bin. 2009

[18]Gene SGL, encoding a kinesin-like protein with transactivation activity, is involved in grain length and plant height in rice. Wu, Tao,Shen, Yingyue,Zheng, Ming,Yang, Chunyan,Chen, Yilin,Feng, Zhiming,Liu, Xi,Liu, Shijia,Jiang, Ling,Wan, Jianmin,Chen, Zhijun,Lei, Cailin,Wang, Jiulin,Wan, Jianmin. 2014

[19]Cloning and characterization of GbGI, a DELLA-like gene from cotton (Gossypium barbadense). Yu, Xiaoling,Ruan, Mengbin,Wen, Wei,Wang, Shuchang,Peng, Ming,Yu, Xiaoling,Cui, Baiming,Di, Rong.

[20]Comparative Transcriptomic Analysis of Two Brassica napus Near-Isogenic Lines Reveals a Network of Genes That Influences Seed Oil Accumulation. Wang, Jingxue,Li, Chen,Yuan, Ling,Singh, Sanjay K.,Pattanaik, Sitakanta,Yuan, Ling,Du, Chunfang,Fan, Jianchun. 2016

作者其他论文 更多>>

微信小程序