Distribution Characteristics of Alleles of Classical SLA-I and II Genes and Bioinformatic Analysis of Novel Alleles in Guizhou Miniature Pigs

文献类型: 外文期刊

第一作者: Chen, Jiajia

作者: Chen, Jiajia;Liu, Zizhan;Wang, Zaigui;Chen, Jiajia;Liu, Zizhan;Yang, Shulin;Li, Kui

作者机构:

关键词: SLA;xenotransplantation;breeding;gene frequency;homology

期刊名称:PAKISTAN JOURNAL OF ZOOLOGY ( 影响因子:0.831; 五年影响因子:0.768 )

ISSN: 0030-9923

年卷期: 2014 年 46 卷 3 期

页码:

收录情况: SCI

摘要: This article has systematically elucidated the alleles distribution characteristics of classical SLA class I and II genes, and analyzed navel alleles in Guizhou miniature pigs via bioinformatics: The frequency of twenty five allele sequences from seven SLA - I and H genes previously derived in this population were calculated. There existed five alleles in SLA-2, four alleles. in SLA-1, three in. QA, QB1 locus;, and two, in SLA-RA, RB1, respectively. The dominant alleles were evident; every locus had 1 or 2 dominant alleles; one in. SLA-3, two in SLA-1 two in QA, SLA-RA and SLA-RB1 loci have only two alleles.. Their distribution is relatively uniform. The comparison between novel SLA class I and II gene sequences. and reference, sequences showed highly polymorphic region of SLA-I focus on the alpha 1 and alpha 2 areas of amino. acid sequence and the polymorphic region of SLA-II mainly expressed in alpha 1 area. The novel alleles and the referenced sequences showed more than 92% homology in amino acid level. The highest amino acid homology percentage of novel alleles and HLA alleles in NCBI were more than 70%. Novel alleles have some functional sites of HLA. According to the analysis. Guizhou miniature pigs have obvious SLA characteristics, it could be the evidences for further haplotypes selection, pig breeding and xenotransplantation.

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