Halomonas songnenensis sp nov., a moderately halophilic bacterium isolated from saline and alkaline soils

文献类型： 外文期刊

第一作者： Jiang, Juquan

作者： Jiang, Juquan;Pan, Yuanyuan;Hu, Baozhong;Huang, Haipeng;Hong, Shan;Meng, Jing;Li, Cheng;Wang, Kaibiao;Jiang, Juquan;Pan, Yuanyuan;Hu, Baozhong;Huang, Haipeng;Hong, Shan;Meng, Jing;Li, Cheng;Wang, Kaibiao;Hu, Shaoxin;Zhang, Xiaoxia

作者机构：

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2014 年 64 卷

页码：

收录情况： SCI

摘要： A moderately halophilic bacterium (strain NEAU-ST10-39(T)) was isolated from saline and alkaline soils in the oilfield of Daqing City, Heilongjiang Province, China. The strain was strictly aerobic, Gram-stain-negative, rod-shaped and motile by peritrichous flagella. Its colonies were yellow. It grew at NaCl concentrations of 0.2-15% (w/v) (optimum 4%, w/v), at temperatures of 4-40 degrees C (optimum 35 degrees C) and at pH 5-10 (optimum pH 7). It did not produce acids from sugars or alcohols. Its DNA G+C content was 57.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD gene sequences indicated that it belonged to the genus Halomonas in the class Gammaproteobacteria. The most phylogenetically related species were Halomonas axialensis, Halomonas meridiana and Halomonas aquamarina, whose types shared 98.3% (16S rRNA), 82.7% (gyrB) and 83.9-84.5% (rpoD) sequence similarity with strain NEAU-ST10-39T. The results of DNA-DNA hybridization assays showed 20 +/- 2 %-50 +/- 1 % relatedness between strain NEAU-ST10-39(T) and the most closely related species including Halomonas axialensis DSM 15723(T), Halomonas meridiana DSM 5425(T), Halomonas aquamarina DSM 30161(T), Halomonas johnsoniae DSM 21197(T), Halomonas stevensii DSM 21198(T), Halomonas nanhaiensis CCTCC AB 2012911(T), Halomonas hamiltonii DSM 21196(T) and Halomonas arcis CGMCC 1.6494(T). The major fatty acids were C-18:1 omega 7c (47.2%), C-16:1 omega 7c and/or C-16:1 omega 6C (18.9 %) and C-16:0 (16.3 %), the only respiratory quinone detected was ubiquinone 9 and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. The new isolate is proposed to represent a novel species with the name Halomonas songnenensis sp. nov., NEAU-ST10-39(T) (=CGMCC 1.12152(T)=DSM 25870(T)) being the type strain.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序