Fungal elicitor protein PebC1 from Botrytis cinerea improves disease resistance in Arabidopsis thaliana

文献类型: 外文期刊

第一作者: Yang, Xiufen

作者: Yang, Xiufen;Zeng, Hongmei;Guo, Lihua;Yuan, Jingjing;Qiu, Dewen

作者机构:

关键词: Arabidopsis thaliana;Botrytis cinerea phytopathogen;Disease resistance;Defense response;Drought tolerance;PebC1 protein;Protein elicitor

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

ISSN: 0141-5492

年卷期: 2014 年 36 卷 5 期

页码:

收录情况: SCI

摘要: We previously identified a novel protein elicitor, PebC1, from Botrytis cinerea and described its enhancement of plant growth, drought tolerance and disease resistance in tomato. Here, we have investigated the defense-associated molecular responses in Arabidopsis thaliana after treatment with recombinant PebC1. PebC1 was expressed in Escherichia coli. Recombinant protein treatments improved plant resistance to Botrytis infection and maintained plant defenses for more than 21 days. The purified protein at 10 mu g ml(-1) activated extracellular medium alkalization (pH) and reactive oxygen species and nitric oxide generation and also induced defense gene expression. Arabidopsis mutants that are insensitive to salicylic acid had increased resistance to Botrytis infection after PebC1 treatment but PebC1 did not affect the resistance of mutants with jasmonic acid and ethylene transduction pathways. The results suggest that PebC1 can function as an activator of plant disease resistance and can promote disease resistance to Botrytis in A. thaliana through the ethylene signal transduction pathway.

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