Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp NF1 and its Application in Xylooligosaccharides Production
文献类型: 外文期刊
第一作者: Zheng, Hong-chen
作者: Zheng, Hong-chen;Pei, Hai-sheng;Yan, Zheng;Sun, Jun-she;Sun, Ming-zhe;Lu, Fu-ping;Meng, Ling-cai;Zhang, Xiu-qing;Zeng, Wen-hui;Zhang, Jing-sheng;Hu, Jin-rong;Sun, Jun-she
作者机构:
关键词: Endoxylanase;Paenibacillus sp.;purification;characterization;oat spelt xylan;xylooligosaccharides
期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )
ISSN: 1017-7825
年卷期: 2014 年 24 卷 4 期
页码:
收录情况: SCI
摘要: High levels of extracellular xylanase activity (211.79 IU/mg) produced by Paenibacillus sp. NF1 were detected when it was submerged-cultured. After three consecutive purification steps using Octyl-Sepharose, Sephadex G75, and Q-Sepharose columns, a thermostable xylanase (XynNF) was purified to homogeneity and showed a molecular mass of 37 kDa according to SDS-PAGE. The specific activity of the purified XynNF was up to 3,081.05 IU/mg with a 14.55-fold purification. The activity of XynNF was stimulated by Ca2+, Ba2+, DTT, and beta-mercaptoethanol, but was inhibited by Fe3+, Zn2+, Fe2+, Cu2+, SDS, and EDTA. The purified XynNF displayed a greater affinity for oat spelt xylan with the maximal enzymatic activity at 60 degrees C and pH 6.0. XynNF, which was shown to be cellulose-free, with high stability at high temperature (70 degrees C-80 degrees C) and low pH range (pH 4.0-7.0), is potentially valuable for various industrial applications. The enzyme hydrolyzed oat spelt xylan to yield mainly xylooligosaccharides (95.8%) of 2-4 degree of polymerization (DP2-4). Moreover, the majority of the xylooligosacharides (DP2-4) products was xylobiose (61.5%). The thermostable xylanase (XynNF) thus seems potentially usefull in the production of xylooligosaccharides.
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