The citrus flavonoid nobiletin inhibits proliferation and induces apoptosis in human pancreatic cancer cells in vitro

文献类型: 外文期刊

第一作者: Zhang, Yu

作者: Zhang, Yu;Li, Hongjun;He, Zhifei;Zhang, Yu;Dou, Huating;Wu, Houjiu

作者机构:

关键词: nobiletin;pancreatic cancer;cell proliferation;apoptosis;cell cycle

期刊名称:FOOD SCIENCE AND BIOTECHNOLOGY ( 影响因子:2.391; 五年影响因子:2.108 )

ISSN: 1226-7708

年卷期: 2014 年 23 卷 1 期

页码:

收录情况: SCI

摘要: The cellular effects of nobiletin on human pancreatic cancer cells (PANC-1) and the mechanisms by which nobiletin inhibits the proliferation of these cells were investigated. A MTT assay and flow cytometry were used to examine cell proliferation and apoptosis, respectively. A Western blot assay was used to examine expression levels of the apoptotic proteins bax, bcl-2, and p53. NOB induced apoptosis in these cells via up-regulation of the proapoptotic protein bax and down-regulation of the antiapoptotic proteins bcl-2 and p53. The normal cell cycle of PANC-1 cells was arrested by NOB with a significant increase in the proportion of G0/G1 phase cells (p < 0.05) and a significant decrease in the proportion of S phase cells (p < 0.05). NOB can inhibit the proliferation of human pancreatic carcinoma cells by inducing apoptosis and arresting the cell cycle progression.

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