Inhibitory effect and mechanism of mesenchymal stem cells on liver cancer cells

文献类型： 外文期刊

第一作者： Hou, Lingling

作者： Hou, Lingling;Wang, Xiaoyu;Zhou, Yaqiong;Ma, Haibin;Wang, Ziling;He, Jinsheng;Hu, Honggang;Guan, Weijun;Ma, Yuehui

作者机构：

关键词： Mesenchymal stem cells;Liver cancer cells;Inhibition;Mechanism

期刊名称：TUMOR BIOLOGY （ 影响因子：3.65； 五年影响因子：3.445 ）

ISSN： 1010-4283

年卷期： 2014 年 35 卷 2 期

页码：

收录情况： SCI

摘要： Mesenchymal stem cells (MSCs), with their capacity for self-renewal and differentiation into various cell types, are important seed cells for stem cell therapy. MSCs exhibit potent pathotropic migratory properties that make them attractive for use in tumor prevention and therapy. However, little is known about the underlying molecular mechanisms that link MSCs to the targeted tumor cells. This study investigated the inhibitory effect and mechanism of MSCs on human hepatoma HepG(2) cells using co-culture and conditioned medium system and animal transplantation model. The HepG(2) cells were co-cultured with MSCs or treated with conditional media derived from MSCs cultures in vitro. Results of methylthiazolyldiphenyl tetrazolium assay and flow cytometric assay showed that the proliferation and apoptosis of HepG(2) cells decreased and increased, respectively. Reverse transcription polymerase chain reaction analysis showed that the expression levels of bcl-2, c-Myc, beta-catenin, and survivin were downregulated. The results of enzyme-linked immunosorbent assay and Western blot proved that MSCs secreted Dkk-1 to inhibit the expression of Wnt signaling pathway-related factors (bcl-2, c-Myc, beta-catenin, and survivin) in tumor cells, consequently inhibiting the proliferation and promoting the apoptosis of HepG(2) cells. Animal transplantation experiment showed that tumor growth was significantly inhibited when HepG(2) cells were co-injected with MSCs into nude mice. These results suggested that MSCs inhibited the growth and promoted the apoptosis of HepG(2) cells in a dose-dependent manner. This study provided a new approach and experimental basis for cancer therapy. This study also proved that the Wnt signaling pathway may have a function in MSC-mediated tumor cell inhibition.

分类号：

  • 相关文献

[1]The Inhibitory Effect of Mesenchymal Stem Cells with rAd-NK4 on Liver Cancer. Cai, Chao,Hou, Lingling,Zhang, Jingsi,Zhao, Diandian,Wang, Ziling,Hu, Honggang,He, Jinsheng,Guan, Weijun,Ma, Yuehui.

[2]Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium. He, ZQ,Fu, YP,Si, HM,Hu, GC,Zhang, SH,Yu, YH,Sun, ZX.

[3]MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells. Bai, Chunyu,Gao, Yuhua,Li, Xiangchen,Wang, Kunfu,Xiong, Hui,Shan, Zhiqiang,Zhang, Ping,Wang, Wenjie,Guan, Weijun,Ma, Yuehui. 2017

[4]Isolation and characterization of mesenchymal stem cells from chicken bone marrow. Bai, Chunyu,Ma, Yuehui,Chen, Lina,Guan, Weijun,Bai, Chunyu,Zhang, Minghai,Hou, Lingling. 2013

[5]Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration. Li, Yang,Liu, Ming-yuan,Wang, Xue-lin,Han, Wen-yu,Liu, Ya-hui,Suo, Jian,Li, Zhi-jie,Li, Ya-gang,Jin, Hu. 2013

[6]Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous Mats. Luo, Yu,Shen, He,Cao, Yuhua,Huang, Jie,Zhang, Mengxin,Dai, Jianwu,Zhang, Zhijun,Luo, Yu,Shi, Xiangyang,Fang, Yongxiang,Dai, Jianwu,Fang, Yongxiang. 2015

[7]MicroRNA-34c acts as a bidirectional switch in the maturation of insulin-producing cells derived from mesenchymal stem cells. Bai, Chunyu,Yang, Wancai,Gao, Yuhua,Zhang, Xiangyang,Bai, Chunyu,Gao, Yuhua,Guan, Weijun,Yang, Wancai. 2017

[8]Wip1 knockout inhibits the proliferation and enhances the migration of bone marrow mesenchymal stem cells. Tang, Yiting,Sheng, Ming,Liu, Honglin,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Xiong, Kai. 2015

[9]Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells. Bai, Chunyu,Gao, Yuhua,Li, Qian,Feng, Yuan,Yu, Yanze,Meng, Gentong,Zhang, Minghai,Bai, Chunyu,Gao, Yuhua,Li, Qian,Guan, Weijun.

[10]Biological characterization of mesenchymal stem cells from bovine umbilical cord. Xiong, Hui,Bai, Chunyu,Wu, Shuang,Gao, Yuhua,Lu, Taofeng,Guan, Weijun,Ma, Yuehui,Xiong, Hui,Hu, Qingyun.

[11]Differentiation potential of bone marrow mesenchymal stem cells in duck. Li, Linfeng,Gong, Xuelian,Liu, Hongkun,Chen, Lina,Guan, Weijun,Ma, Yuehui,Li, Linfeng,Bai, Xiujuan. 2009

[12]Allelopathic effects of allelochemicals of Ginkgo biloba leaf on fusarium wilt (Fusarium oxysporum) of hot pepper. Hou, Y. X.,Song, X. Y.,Yin, Y. L.,Li, Y. S.,Yang, J. S.,Zheng, J. Y.,Yin, Y. L.. 2016

[13]Joint Application of Isaria fumosorsea Chitinase and Azadirachtin as Biopesticides against the Diamondback Moth, Plutella xylostella L. (Lepidoptera: Plutellidae). Long, X. Z.,Yu, Y. H.,Huang, T. T.,Ali, S.. 2016

[14]Inhibiting sulfate reducing bacteria activity by denitrification in an anaerobic baffled reactor: influencing factors and mechanism analysis. Zhang, Zhaohan,Feng, Yujie,Ren, Nanqi,Zhang, Zhaohan,Liu, Guangmin,Chen, Zhongxi,Zhang, Zhaohan,Zhan, Peirong. 2014

[15]Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection. Ishag, Hassan Z. A.,Li, Chen,Wang, Fengjuan,Mao, Xiang,Mao, Xiang,Ishag, Hassan Z. A..

[16]Fusarium graminearum growth inhibition due to glucose starvation caused by osthol. Shi, Zhiqi,Shen, Shouguo,Zhou, Wei,Wang, Fei,Fan, Yongjian. 2008

[17]Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid. Zhang, Lan,Zhang, Yanning,Zhang, Pei,Jiang, Hongyun.

[18]Toxicity of Lanthanum to Pathogenic Fungi and Its Morphological Characteristics. Mu Kangguo,Zhao Xiuqin,Hu Lin,Zhang Fusuo,Zhang Wenji,Cui Jianyu.

[19]Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Li, Guo-Xin,Tian, Zhi-Jun,Yu, Hai,Jin, Yuan-Yuan,Hou, Shao-Hua,Zhou, Yan-Jun,Liu, Tian-Qiang,Hu, Shou-Ping,Tong, Guang-Zhi,Li, Guo-Xin,Tong, Guang-Zhi,Li, Guo-Xin,Yu, Hai,Zhou, Yan-Jun,Tong, Guang-Zhi.

[20]Aflatoxin B-1 inhibition in Aspergillus flavus by Aspergillus niger through down-regulating expression of major biosynthetic genes and AFB(1) degradation by atoxigenic A. flavus. Xing, Fuguo,Wang, Limin,Liu, Xiao,Selvaraj, Jonathan Nimal,Wang, Yan,Zhao, Yueju,Liu, Yang.

作者其他论文 更多>>

微信小程序