Nitazoxanide inhibits the replication of Japanese encephalitis virus in cultured cells and in a mouse model

文献类型： 外文期刊

第一作者： Shi, Zixue

作者： Shi, Zixue;Wei, Jianchao;Deng, Xufang;Qiu, Yafeng;Shao, Donghua;Li, Beibei;Zhang, Keyu;Xue, Feiqun;Ma, Zhiyong;Li, Shuqing;Wang, Xiaodu

作者机构：

关键词： Japanese encephalitis virus;Nitazoxanide (NTZ);Antiviral

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2014 年 11 卷

页码：

收录情况： SCI

摘要： Background: Japanese encephalitis virus (JEV) has a significant impact on public health. An estimated three billion people in 'at-risk' regions remain unvaccinated and the number of unvaccinated individuals in certain Asian countries is increasing. Consequently, there is an urgent need for the development of novel therapeutic agents against Japanese encephalitis. Nitazoxanide (NTZ) is a thiazolide anti-infective licensed for the treatment of parasitic gastroenteritis. Recently, NTZ has been demonstrated to have antiviral properties. In this study, the anti-JEV activity of NTZ was evaluated in cultured cells and in a mouse model. Methods: JEV-infected cells were treated with NTZ at different concentrations. The replication of JEV in the mock-and NTZ-treated cells was examined by virus titration. NTZ was administered at different time points of JEV infection to determine the stage at which NTZ affected JEV replication. Mice were infected with a lethal dose of JEV and intragastrically administered with NTZ from 1 day post-infection. The protective effect of NTZ on the JEV-infected mice was evaluated. Findings: NTZ significantly inhibited the replication of JEV in cultured cells in a dose dependent manner with 50% effective concentration value of 0.12 +/- 0.04 mu g/ml, a non-toxic concentration in cultured cells (50% cytotoxic concentration = 18.59 +/- 0.31 mu g/ml). The chemotherapeutic index calculated was 154.92. The viral yields of the NTZ-treated cells were significantly reduced at 12, 24, 36 and 48 h post-infection compared with the mock-treated cells. NTZ was found to exert its anti-JEV effect at the early-mid stage of viral infection. The anti-JEV effect of NTZ was also demonstrated in vivo, where 90% of mice that were treated by daily intragastric administration of 100 mg/kg/day of NTZ were protected from a lethal challenge dose of JEV. Conclusions: Both in vitro and in vivo data indicated that NTZ has anti-JEV activity, suggesting the potential application of NTZ in the treatment of Japanese encephalitis.

分类号：

  • 相关文献

[1]LiCl inhibits PRRSV infection by enhancing Wnt/beta-catenin pathway and suppressing inflammatory responses. Hao, Hong-ping,Li, Jia-rong,Wang, Yue,Ni, Bo,Wang, Rui,Wang, Xin,Sun, Ming-xia,Fan, Hong-jie,Mao, Xiang,Wen, Li-bin,Fan, Hong-jie. 2015

[2]Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro. Li, Wenliang,Mao, Li,Yang, Leilei,Hao, Fei,Zhang, Wenwen,Jiang, Jieyuan,Cao, Yongguo,Zhou, Bin,Han, Linxiao,Lin, Tao. 2017

[3]Lentivirus-mediated RNA interference against Japanese encephalitis virus infection in vitro and in vivo. Shen, Ting,Cao, Ruibing,Zhou, Bin,Chen, Puyan,Liu, Ke,Miao, Denian.

[4]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[5]mosGCTL-7, a C-Type Lectin Protein, Mediates Japanese Encephalitis Virus Infection in Mosquitoes. Liu, Ke,Qian, Yingjuan,Jung, Yong-Sam,Zhou, Bin,Cao, Ruibing,Shen, Ting,Chen, Puyan,Zhu, Huaimin,Liu, Ke,Shao, Donghua,Wei, Jianchao,Ma, Zhiyong,Qiu, Yafeng.

[6]Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection. Ishag, Hassan Z. A.,Li, Chen,Wang, Fengjuan,Mao, Xiang,Mao, Xiang,Ishag, Hassan Z. A..

[7]Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China. Fan, Jian-Ming,Luo, Jun,Teng, Man,Bu, Dan,Wang, Fang-Yu,Wang, Li,Zhang, Gai-Ping,Fan, Jian-Ming,Chen, Lu,Wang, Chuan-Qing. 2010

[8]Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate. Hua, Rong-Hong,Li, Ye-Nan,Chen, Zhen-Shi,Liu, Li-Ke,Huo, Hong,Wang, Xiao-Lei,Guo, Li-Ping,Shen, Nan,Wang, Jing-Fei,Bu, Zhi-Gao. 2014

[9]Porcine alpha interferon inhibit Japanese encephalitis virus replication by different ISGs in vitro. Liao, Xuewen,Yao, Huijuan,Fan, Shengchao,Miao, Denian,Liu, Ke,Zhou, Bin,Chen, Puyan,Liu, Ke. 2013

[10]A simple method for developing an infectious cDNA clone of Japanese encephalitis virus. Zheng, Hao,Zheng, Xuchen,Tong, Wu,Liu, Fei,Liang, Chao,Wang, Tao,Gao, Fei,Li, Liwei,Shan, Tongling,Li, Guoxin,Tong, Guangzhi,Zheng, Hao,Tong, Wu,Tong, Guangzhi.

[11]Antibodies generated by immunization with the NS1 protein of West Nile virus confer partial protection against lethal Japanese encephalitis virus challenge. Sun, EnCheng,Zhao, Jing,TaoYang,Xu, QingYuan,Qin, YongLi,Wang, WenShi,Wei, Peng,Wu, DongLai. 2013

[12]Molecular characterization of Japanese encephalitis virus strains prevalent in Chinese swine herds. Zheng, Hao,Shan, Tongling,Deng, Yu,Sun, Chunqing,Yuan, Shishan,Yin, Yang,Tong, Guangzhi. 2013

[13]Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice. Wu, Rui,Tian, Yongxiang,Deng, Junhua,Yang, Keli,Liang, Wangwang,Guo, Rui,Duan, Zhengying,Liu, Zewen,Zhou, Danna,Xu, Diping,Wu, Rui,Tian, Yongxiang,Xu, Diping,Wu, Rui. 2011

[14]Genetic instability of Japanese encephalitis virus cDNA clones propagated in Escherichia coli. Zheng, Xuchen,Tong, Wu,Liu, Fei,Liang, Chao,Gao, Fei,Li, Guoxin,Tong, Guangzhi,Zheng, Hao,Tong, Guangzhi,Zheng, Hao.

[15]Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Tong, Guang-Zhi,Bin Wang,Hua, Rong-Hong,Tian, Zhi-Jun,Chen, Na-Sha,Zhao, Fu-Rong,Liu, Tian-Qiang,Wang, Yun-Feng,Tong, Guang-Zhi. 2009

[16]Antigenic epitopes mapping and functional analysis of domain III of Japanese encephalitis virus envelope protein. Yan Li-Ping,Hua Rong-Hong,Tong Guang-Zhi,Yan Li-Ping,Qi Wen-Bao. 2008

[17]MicroRNA transcriptome profiling of mice brains infected with Japanese encephalitis virus by RNA sequencing. Li, Xin-Feng,Cao, Rui-Bing,Wang, Jing-Man,Zhang, Yuan-Peng,Gu, Jin-Yan,Feng, Xiu-Li,Zhou, Bin,Chen, Pu-Yan,Luo, Jun,Fan, Jian-Ming.

[18]Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections. Yang, Zhenhua,Wu, Rui,Li, Ling,Guo, Deyin,Pan, Zishu,Li, Robert W.,Xiong, Zhongliang,Zhao, Haizhong.

作者其他论文 更多>>
  • An Overview of Zika Virus and Zika Virus Induced Neuropathies

    作者：Wahaab, Abdul;Tran Nguyen Minh, Hieu;Rasgon, Jason L.;Wahaab, Abdul;Tran Nguyen Minh, Hieu;Rasgon, Jason L.;Wahaab, Abdul;Tran Nguyen Minh, Hieu;Rasgon, Jason L.;Wahaab, Abdul;Tran Nguyen Minh, Hieu;Rasgon, Jason L.;Mustafa, Bahar E.;Mustafa, Bahar E.;Tawaab, Abdul;Hameed, Muddassar;Hameed, Muddassar;Hameed, Muddassar;Batool, Hira;Shoaib, Anam;Wei, Jianchao

    关键词：neurotropic flaviviruses; zika virus; neuropathies; transmission; neuropathogenesis; prevention; vaccines; treatment; antivirals; mosquito/vector control; oncolytic; animal and cell models

  • The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew

    作者：Guo, Guanghao;Hou, Yikun;Zhang, Huaizhi;Lu, Ping;Li, Miaomiao;Dong, Lingli;Xie, Jingzhong;Zhu, Keyu;Li, Beibei;Dong, Lei;Yang, Yijun;Qiu, Dan;Wang, Gaojie;Zhao, Yusheng;Chen, Yuhang;Liu, Zhiyong;Guo, Guanghao;Ahn, Hee-Kyung;Zhao, He;Jones, Jonathan D. G.;Bai, Kaihong;Hou, Yikun;Li, Beibei;Dong, Lei;Liu, Zhiyong;Gong, Zhen;Han, Guan-Zhu;Wu, Qiuhong;Chen, Yongxing;Li, Wenling;Li, Hongjie;Zhang, Panpan;Yuan, Chengguo;Shi, Wenqi;Xue, Minfeng;Yang, Lijun;Yu, Dazao;Hu, Tiezhu;Liu, Zhiyong;Ahn, Hee-Kyung

    关键词：wheat; powdery mildew; NLR pair; hetero-complexes; resistance mechanism

  • Discovery of xanthone-based nitric oxide donors targeting biofilm clearance

    作者：Tang, Qun;Ye, Wenchong;Lv, Sai;Zhang, Keyu;Han, Xiangan;Wang, Chunmei;Wang, Xiaoyang;Zhou, Wen;Tang, Qun;Ye, Wenchong;Lv, Sai;Zhang, Keyu;Wang, Chunmei;Wang, Xiaoyang;Zhou, Wen;Chandarajoti, Kasemsiri;Chandarajoti, Kasemsiri;Ge, Rile;Bai, Han

    关键词：biofilm clearance; biofilm infection; nitric oxide (NO); alpha-mangostin-based NO donors

  • Development of a Noninfectious Japanese Encephalitis Virus Replicon for Antiviral Drug Screening and Gene Function Studies

    作者：Yang, Yang;Zheng, Jiayang;Lin, Yafang;Zhang, Hailong;Zhang, Junjie;Li, Zongjie;Liu, Ke;Li, Beibei;Shao, Donghua;Qiu, Yafeng;Ma, Zhiyong;Wei, Jianchao;Zhang, Yan;Zhao, Qianming

    关键词：Japanese encephalitis virus; replicon; green fluorescent protein; antiviral drug screening; RNA replication

  • Sheep serve as amplifying hosts of Japanese encephalitis virus, increasing the risk of human infection

    作者：Zhang, Hailong;Li, Dan;Zheng, Jiayang;Qiu, Yafeng;Liu, Ke;Li, Zongjie;Li, Beibei;Shao, Donghua;Ma, Zhiyong;Wei, Jianchao;Zhang, Hailong;Liu, Juxiang;Bao, Jingyue;Wang, Zhiliang

    关键词：

  • Partial protective efficacy of the current licensed Japanese encephalitis live vaccine against the emerging genotype I Japanese encephalitis virus isolated from sheep

    作者：Zhang, Hailong;Liu, Juxiang;Zhang, Yan;Li, Dan;Zheng, Jiayang;Zhang, Junjie;Li, Zongjie;Liu, Ke;Li, Beibei;Shao, Donghua;Qiu, Yafeng;Ma, Zhiyong;Wei, Jianchao

    关键词：Japanese encephalitis virus; sheep; SA14-14-2 vaccine; neutralizing antibodies; protective efficacy

  • Preparation and functional identification of various porcine cytokines

    作者：Yang, Shanshan;Cao, Qiuxia;Yan, Kexin;Wang, Chuanhong;Song, Xu;Bian, Xianyu;Li, Sufen;Cheng, Zhenkong;Zhang, Xuehan;Wang, Yi;Guo, Rongli;Fan, Baochao;Li, Bin;Yang, Shanshan;Guo, Rongli;Fan, Baochao;Li, Bin;Yang, Shanshan;Guo, Rongli;Fan, Baochao;Li, Bin;Cao, Qiuxia;Wang, Xiaodu;Song, Houhui

    关键词：Mucosal immune response; Protein purification; Functional identification; Porcine cytokines; Porcine epidemic diarrhea; Porcine intestinal organoids

微信小程序