Resiquimod and polyinosinic-polycytidylic acid formulation with aluminum hydroxide as an adjuvant for foot-and-mouth disease vaccine

文献类型: 外文期刊

第一作者: Zhou, Chun-Xue

作者: Zhou, Chun-Xue;Li, Dong;Chen, Ying-Li;Lu, Zeng-Jun;Sun, Pu;Cao, Yi-Mei;Bao, Hui-Fang;Fu, Yuan-Fang;Li, Ping-Hua;Bai, Xing-Wen;Xie, Bao-Xia;Liu, Zai-Xin

作者机构:

关键词: R848;Poly(I:C);Aluminum hydroxide;FMDV

期刊名称:BMC VETERINARY RESEARCH ( 影响因子:2.741; 五年影响因子:2.955 )

ISSN: 1746-6148

年卷期: 2014 年 10 卷

页码:

收录情况: SCI

摘要: Background: Toll-like receptor (TLR) agonists reportedly have potent antiviral and antitumor activities and may be a new kind of adjuvant for enhancing immune efficacy. Resiquimod (R848) is an imidazoquinoline compound with potent antiviral activity and functions through the TLR7/TLR8 MyD88-dependent signaling pathway. Polyinosinic-polycytidylic acid [poly(I:C)] is a synthetic analog of double-stranded RNA that induces the production of pro-inflammatory cytokines by the activation of NF-kappa B through TLR3. This study investigated the potential of R848 and poly(I:C) as an adjuvant 146S foot-and-mouth disease virus (FMDV) vaccine formulated with aluminum hydroxide (Al(OH)(3)). Results: Antibody titers to FMDV and CD8(+) T cells were markedly enhanced in mice immunized to 146S FMDV + Al(OH)(3) + R848 + poly(I:C) compared with mice immunized to FMDV + ISA206. IFN-gamma secretion substantially increased compared with IL-4 secretion by splenic T cells stimulated with FMDV antigens in vitro, suggesting that R848, poly(I:C), and with Al(OH)(3) together biased the immune response toward a Th1-type direction. Conclusions: These results indicated that the R848 and poly(I:C) together with Al(OH)(3) enhanced humoral and cellular immune responses to immunization with 146S FMDV antigens. Thus, this new vaccine formulation can be used for FMDV prevention.

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